44 | 405 TS Getting Started Guide
Option Description/Values range
Default
values
Aspirate
Vacuum Filtration: Select standard aspiration (Top)
or Vac for filtration.
Filtration
Time:
When applicable, specify duration of vacuum filtration
in seconds. 5-999
30
Travel
Rate:
The rate at which the washer manifold travels down
into the wells. The selection range is 1 to 5 for non-
cell-based assays, from slowest to fastest. With
these rates, the tubes slow their descent as they
approach the defined aspirate height (Z Position) to
aid complete evacuation of the well.
3
For delicate, cell-based assays, the range is 1CW
(cell wash) to 4CW and 6CW. These rates minimize
turbulence in the wells. The tubes descend at a
constant rate to the specified height. Rate 6CW
creates the least disturbance and performs fastest.
Delay: Amount of time the tubes stay at the aspirate height
before lifting out of the wells. Define a delay between
0 - 5000 ms. Increasing the delay may improve
evacuation of the wells.
0
Positioning: X- and Y- horizontal axes, Z- height (vertical) axis
can be adjusted to improve performance. Default Z-
axis for 384-well plates is 22 steps, 2 for 384-well
PCR plates.
Z = 29 for
96-well
plates;
X & Y = 0
Secondary
aspirate:
Also called Crosswise Aspiration. First the wells are
aspirated using the position defined above. The
aspirate tubes rise and then descend to the
secondary position to aspirate again.
No
Dispense
Flow Rate:
96-tube &
192-tube
manifolds
The rate at which the fluid is dispensed from the
tubes. For cell-based assays, use rate 1 or 2 for
gentle washing with the 96-tube manifold only. For
normal dispensing, the range is 3-11, 3 is slowest and
11 is fastest.
7
405™ TS Microplate Washer
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