Predefined Sample Protocols| 45
Option Description/Values range
Default
values
Volume: µL/well dispensed range:
96-tube manifold: 50-3000
192-tube manifold: 25-3000
µL/well:
96= 300
384= 100
Buffer: Buffer bottle selection. A-D A
Positioning: X- and Y- horizontal axes, Z- height (vertical) axis
can be adjusted to improve performance. Default Z-
axis for 384-well plates is 120 steps; 83 for 384-well
PCR plates.
Z = 121 for
96-well
plate
Vacuum
Delay
Suspends the vacuum pump until a certain volume is
dispensed. This feature is critical to cell wash
operations. It delays normal aspiration until the
specified volume has been dispensed to the wells.
The range is 10 to 350 µL/well.
10
Pre-
dispense
Quick, small prime to condition the tips before
dispensing.
No
Flow Rate: 96-tube & 192-tube manifolds: The range is 3-11, 3 is
slowest and 11 is fastest.
9
Volume: µL/tube dispensed range:
96-tube manifold: 50-3000
192-tube manifold: 25-3000
µL/tube:
96 = 50
192 = 25
Bottom
wash
Bottom washing adds an initial wash cycle to the
specified number of cycles. Fluid is simultaneously
dispensed and aspirated to create cleaning
turbulence (at the specified height). The manifold
descends to aspirate again and ends with a final
dispense to fill the wells.
No
Rate: Valid range is 3-11. The cell wash rates, 1 CW and 2
CW, which use low-flow tubing, are available but not
recommended. Cell wash options are designed for
gentle washing, while bottom wash is designed for
vigorous washing.
Wash
Volume:
25-3000 µL/well dispense 250
BioTek Instruments, Inc.
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