Washer Problems| 97
Problem Possible Cause What To Do
Uneven
aspiration of
water buffer.
Some wells
left full.
No surfactant in
the buffer, such as
Tween
®
20.
Add surfactant to the buffer. If this is not
possible, continue below.
Insufficient
vacuum.
BioTek offers a high-flow pump for assays
using only water for the wash fluid. Contact
BioTek for more information.
Protocol settings
not optimized.
Optimize protocols to improve evacuation
Aspiration tubes
not properly
positioned
horizontally in
wells.
If none of the tubes are bent, try adjusting
the horizontal aspirate position (X-/Y-axis) in
the protocol.
Microplate not level
in carrier, or strips
not level in holder.
Reseat microplate in carrier or strips in
holder.
Make sure the carrier is clean.
Try a different microplate or strip holder.
If the problem is unresolved, the carrier may
have to be realigned. Contact BioTek TAC.
Too much
residual left in
wells after
aspiration.
Clogged vacuum
filter.
If you are using an in-line vacuum filter (PN
49943), the filter may need to be cleaned or
replaced.
Waste bottle cover
not properly sealed
or fittings not
properly
connected.
Firmly seat the waste bottle stopper. Make
sure tubing is connected properly.
Manifold out of
alignment or not
moving freely.
Check for obstructions. If none are found,
contact BioTek TAC.
Protocol requires
optimization.
Optimize protocols to improve evacuation.
Aspirate tubes are
bent.
Contact BioTek TAC.
BioTek Instruments, Inc.
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