Maintenance 5-2
Weak Detergent Cleaning
To clean the pump with weak detergent, follow these steps:
1 Prime the pump with a weak detergent solution (e.g., 2% solution of CONTRAD®,
RoboScrub, or flo-kleen) and allow the solution to remain in the pump with the
syringe fully lowered for 30 minutes.
2 After the 30-minute period, remove the reagent tubing from the detergent and cycle
all the fluid from the syringe and tubing into a waste container.
3 Prime the pump a minimum of 10 cycles with distilled or deionized water. Leave the
fluid pathways filled for storage.
CONTRAD can be purchased through Curtis Matheson Scientific, Inc. Order P/N 117-
655 for 500 mL syringe size.
flo-kleen can be purchased through Ciba Corning Gilford Systems. Order P/N 402342
for 400 mL syringe size.
RoboScrub is a phosphate-free detergent for cleaning and conditioning liquid handling
systems. RoboScrub rinses away chemicals, solvents, blood, and corrosive acids.
Weak Acid-Base-Sequence Cleaning
To clean the pump with weak acid and base, follow these steps:
1 Prime the pump with 0.1 N NaOH and allow the solution to remain in the pump(s)
for 10 minutes with the syringes fully lowered.
2 Flush the pump with distilled or deionized water.
3 Prime the pump with 0.1 N HCl, and allow the solution to remain in the pump for 10
minutes with the syringes fully lowered.
4 After a 10-minute period, remove the reagent tubing from 0.1 N HCl solution and
cycle all the fluid from the syringes and tubing into a waste container.
5 Prime the pump a minimum of 10 cycles with distilled or deionized water.
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