Maintenance 5-6
Replacing the Reagent Syringe Seals
NOTE See Chapter 2, “Hardware Setup,” for an illustration of the syringe
components.
To replace the reagent syringe seals, follow these steps:
1 Remove the syringe from the pump.
2 Remove the syringe plunger from the barrel.
3 Using a single edged razor or precision knife, carefully slice the old seal lengthwise
and remove it from the plunger. Care must be taken not to damage the plunger or
“O”-rings beneath the seal.
4 Wet the “O”-ring (if present) and plunger tip with distilled or deionized water.
5 Place the seal on a flat surface with the open end facing up. Press the plunger tip
firmly into the hole until it snaps into position.
6 Lay the plunger on a flat table top, and position it so that the seal (from the “O”-ring
up) hangs over the edge.
7 Slowly roll the plunger along the table edge pressing firmly on the portion of the seal
below the “O”-ring. See Figure 5-2.
Figure 5-2. Syringe Seal Assembly
8 Rotate the plunger three complete turns. This is necessary to make the sharp raised
edge of the plunger bite into the seal for a secure fit.
9 Wet the seal with distilled or deionized water, replace the plunger, then replace the
syringe.
NOTE Syringe sizes 250 µL, 500 µL, 1 mL, 2.5 mL, and 5.0 mL have “O”-rings.
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