3.5. Diluting Process
The parts of the fluidics are rinsed with diluent reagent. The measuring chamber is filled up with
1 ml of diluent.
The sampling process has aspirated 25 µl of sample, which is in the sampling needle. In the first
diluting step the sample is dispensed into the measuring chamber with 3 ml of diluent, which
comes from the Macro syringe through V8 (On) and Micro-dilutor, while the M3 Dilutor motor
moves upwards. This process makes the 1:160 first dilution rate in the chamber.
The RBC sample – 25 µl of MIX dilution – is stored in the sampling needle during the WBC
measurement and the cleaning process. The instrument makes the RBC dilution into the
chamber after these processes.
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