Design and function qTOWER iris
14
4.2.4 Plastic
NOTICE
Unsuitable sample vessels and microtiter plates can lead to instrument damage and in-
correct results.
¡ Only use sample vessels and microtiter plates that are suitable for a PCR application.
¡ Seal the samples. Use the sample vessels with lids and adhesive foil for microtiter
plates.
¡ The optical transparency of the foils affects the fluorescence signal directly. Use only
clear adhesive foils and optical lids as offered for real-time PCR.
Sample blocks in 96-well SBS format are suitable for use with 0.2mL individual tubes,
8-well strips and 96-well microtiter plates.
Sample block in 384-well SBS format are only suitable for use with standard PCR plates
with 384 wells.
These and other consumables can be ordered from Analytik Jena.
Regardless of the sealing method used, thanks to the lid technology, the same pressure
is always applied to the consumables for absolutely reproducible conditions.
The device is not limited to specific detection reagents or the plastic products of a spe-
cific manufacturer.
4.2.5 Software
The device can be controlled via the qPCRsoft software on an external PC or an inte-
grated tablet.
Desktop software
The desktop software provides the following functions:
¡ Device control and monitoring
¡ Design of real-time PCR experiments and their evaluation
¡ Storage of methods (templates) and measuring results (projects)
¡ User management
¡ Planning and evaluation of
– Absolute quantifications
– Relative quantifications
– ΔΔCt analyses
– DNA melting curves
– Genotyping
– End point analyses
¡ Results export to MS EXCEL or as CSV file
¡ Results printout
¡ Results export to extended programs for the analysis of real-time PCR data (e.g.
GenEx)