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Endress+Hauser Analytik Jena Biometra TRIO User Manual

Endress+Hauser Analytik Jena Biometra TRIO
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Operating Manual
Biometra TRIO
Multi-Block Thermal Cycler
Three PCR Devices in One

Table of Contents

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Endress+Hauser Analytik Jena Biometra TRIO Specifications

General IconGeneral
TypeThermal Cycler
Heating RateUp to 4.5 °C/s
Cooling RateUp to 3.5 °C/s
Gradient FunctionYes
Gradient Range30 °C to 99 °C
ConnectivityUSB, Ethernet
Power Supply100-240 V, 50/60 Hz
Temperature Uniformity±0.3°C
DisplayTouchscreen

Summary

Basic Information

User Manual Notes and Conventions

Notes on using the manual, conventions, symbols, and signal words for safety.

Intended Use of Biometra TRIO

Describes the Biometra TRIO as an end-point thermal cycler for nucleic acid amplification.

Safety Instructions

General Safety Notes

General safety precautions and compliance with regulations for safe operation.

Device Safety Markings

Explains warning and instruction signs attached to the device for safety.

Function and Setup

Field of Applications

Overview of PCR and the Biometra TRIO's role in amplifying nucleic acids.

General Description of Biometra TRIO

Describes the three models of the Biometra TRIO and its interface.

Installation and Commissioning

Delivery Contents and Unpacking

Lists the items included in the delivery package and instructions for unpacking.

Operation Voltage and Installation Conditions

Guidance on setting voltage and environmental/setup requirements for installation.

Control Elements

Front and Rear Views

Identifies and labels components visible on the front and rear of the Biometra TRIO.

High Performance Smart Lid (HPSL)

Details the features and operation of the height-adjustable heated lid.

Loading Samples

Instructions on how to load samples into the thermal cycler's block.

Operating

Power On Self-Test and Login

Describes the initial self-test and the functions available on the log-in screen.

User Login and Home Screen

Guides through the login process and the features of the home screen interface.

Creating, Editing, and Saving Programs

Programming Modes

Explains the spreadsheet and graphical programming modes for creating PCR protocols.

Creating New Programs and Templates

Guide for creating new programs or using pre-installed templates.

Assigning Program Names

Instructions for naming programs using the virtual keyboard.

Setting Heated Lid Temperature

How to set the temperature and status of the heated lid.

Starting, Copying, and Deleting Programs

Starting Programs

Instructions on how to start previously used or stored programs.

Copying Programs

Steps for copying individual programs to different locations.

Deleting Programs

Procedure for deleting single or all programs from the device.

Running, Pausing, Continuing, and Stopping Programs

Display During Run

How to view the running program in spreadsheet or graphical mode.

Program Control (Pause, Continue, Skip)

Steps to pause, continue, or skip a step in a running program.

Stopping Programs

Procedure for stopping an active program run.

Protocol Wizard

Polymerase Selection and Templates

Describes using polymerase-specific templates to create PCR programs.

Protocol Wizard Parameter Settings

Setting parameters for automatic PCR program creation using the wizard.

Primer Annealing Temperature Calculator

Tool for calculating theoretical primer annealing temperature based on sequences.

Tools

11.1 Instrument Settings

Overview of basic instrument settings like date, time, network, and display.

11.2 User Management

Manages user accounts, rights, and security for the instrument.

11.3 Documentation and Log Files

Accessing log files (Run-Logfile, Power-On-Logfile, Error-Logfile) for instrument data.

11.4 Extended Self-Test Procedure

Performing self-tests to check instrument functions and components.

11.5 Service Info File (SINF) Creation

Creating a service information file for remote failure diagnosis.

11.6 Backup and Restore Functions

Synchronizing and backing up instrument data, programs, and user settings.

11.7 Cycler Information

Displays general information about the instrument hardware and software.

11.8 Contacting Service

Provides contact details for the Biometra service department.

Adaptation of Programs

Short Manual

Initial Operation and Login

Quick guide for initial setup, power-on, and logging into the instrument.

Program Management and Operation

Summary of program creation, editing, saving, and running procedures.

Fault Removal

Forgotten Password

Steps to recover or reset a forgotten administrator or user password.

Slow Heating and Cooling Issues

Troubleshooting tips for slow heating or cooling performance, checking ventilation.

Auto Restart Behavior

Explains how the instrument handles power failures and restarts.

Unrecognized Power Failure Restart

Addresses restarts due to voltage fluctuations and proper connection advice.

Maintenance and Repair

Factory Calibration and Servicing

Information on factory calibration, and when to contact service for repairs.

Cleaning and Disinfection

Procedures for cleaning the housing and sample block, and disinfection methods.

Firmware Update and Spare Parts

Guidance on firmware upgrades and using only original spare parts.

Service

Instructions for Return Shipment

Procedures and requirements for returning the instrument for service.

Packing of the Biometra TRIO Thermal Cycler

Instructions for properly packing the thermal cycler for transport.

Disposal

Technical Specifications

Declaration of Conformity

Index

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