Endress+Hauser Analytik Jena Biometra TRIO - Primer Annealing Temperature Calculator

Biometra TRIO Protocol Wizard
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10.5 Primer Annealing Temperature Calculator
Based on the primer sequences the Biometra TRIO protocol wizard can calculate the
theoretical primer annealing temperature (T
a
). Press the calc Ta button in the Biometra
TRIO protocol wizard parameter screen (see Fig. 43) to access the primer annealing
temperature calculator screen (see Fig. 44).
Calc Ta
In the annealing temperature calculator screen enter the sequences for the forward and
reverse primer using the button below the input fields. The sequence length is counted
by the software and displayed in brackets left to the input fields.
Fig. 46 Biometra TRIO primer annealing temperature calculator screen
To adjust the primer or salt concentration press:
Edit concentration
In the next screen the primer concentration, salt concentration and magnesium concen-
tration can be adjusted. Touch the corresponding input field and enter values using the
keypad. The concentrations have the following default values and ranges:
Function Default value Range
Primer concentration 200 nM 0-2.000 nM
Salt concentration 50 mM 0-200 mM
Magnesium concentration 0 mM 0-200 mM