Biometra TRIO Adaptation of programs
101
12 Adaptation of programs
If programs are exchanged between different thermal cycler models or of the sample
block is exchanged programs might need to be adapted prior to start or during editing.
The following table provides an overview for possible adaptations:
Cause Example Adjustment
Maximum gradient range
is exceeded.
A program for the 96 well gradient silver
block with a programmed temperature
range > 30°C is to be started on a 96
well gradient aluminum block and there-
fore exceeds the block's maximum gradi-
ent range.
The temperature range of the gradient will be re-
duced based on the average temperature of the
maximum permissible value. If the average tem-
perature of the programmed gradient is, for exam-
ple, 60°C and the temperature range is ± 20°C, it
will be reduced to 60°C ± 15°C.
A program including a
gradient step is trans-
ferred to a non- gradient
instrument.
The program is including a gradient step
started on a block without gradient func-
tion.
The gradient will be deleted and the average tem-
perature/annealing temperature will be used for
this step. If the average temperature/annealing
temperature of the programmed gradient is for ex-
ample 60°C, this value will be used for the step.
Gradient out of limit A program for the 96 well silver block
with a programmed gradient tempera-
ture below 20°C is to be started on a 96
well aluminum block and falls below
minimum permitted temperature.
The lower temperature is increased to the mini-
mum permitted value. If for example a gradient is
programmed from 5°C to 25°C the gradient is
changed to 20°C to 25°C.
A program including a
temperature optimization
step (TOS) is transferred
to an instrument that
does not offer this func-
tion
A TOS program is transferred from the
Biometra TRIO to another thermal cycler
The mean value of the programmed temperatures
in the TOS step for the left and right sample block
is used.
The ramping rate exceeds
the limit
A program for the 96 well gradient silver
block is started on a 96 well aluminum
block.
The ramp rate will be adjusted to the maximum
possible ramp rate for the installed block type. If
the programmed ramp rate is for example 8°C/s it
will be reduced to 6°C/s.
The ramping rate is lower
than the maximum
A program for the 96 well aluminum
block is started on a 96 well silver block.
The ramp rate will be adjusted to the maximum
possible ramp rate for the installed block type. If
the programmed ramp rate is for example 6°C/s it
will be increased to 8°C/s.
User defined ramping
rate
The ramping rate for a program step is
lower than maximum value
The user defined value is maintained and does not
become adjusted
Note: If programs need to be adapted to the installed sample block prior to the start or
editing a message will be displayed. The user can confirm or can deny the adaptations. If
during program start the necessary adaptation is denied the program cannot be started
by the instrument.
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