Biometra TRIO Function and setup
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Fig. 1 Biometra TRIO Thermal Cycler
The Biometra TRIO Thermal Cycler features an automatic restart. If a power failure oc-
curs during the run the instrument will continue the run when the power returns. In case
of long- term power failure (longer than 30 minutes) the instrument keeps the sample
block at 4°C(freeze step) and the user can decide to repeat the run with the same sam-
ples or to discard them.
This instruction manual provides information on how to use all models of the Biometra
TRIO Thermal Cycler most effectively.
3.3 Special features
3.3.1 Thermal Block
The Biometra TRIO Thermal Cycler is available with different block formats. The 48 well
version can be used with standard 0.2 ml tubes, strips or plates. With 30 well blocks the
Biometra TRIO can be used with 0.5 ml tubes and the combi block version offers the
flexibility to incubate 0.2 ml tubes, strips or plates or 0.5 ml tubes. With a sample capac-
ity of up to 144 samples in parallel the Biometra TRIO 48 can also be used for high
throughput applications.
By the rubber sealing at the lid an encapsulated space is formed when the lid is closed.
The closed room serves to improve the temperature uniformity of the sample block and
avoids formation of condensed water at the final PCR cooling step. Furthermore all
blocks are perfectly sealed to prevent condensed water from penetrating the Peltier ele-
ments underneath the sample block and other parts of the electronics. By the perfect
sealing the Peltier elements are protected and the instruments life time is expanded.
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