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General| Brand | JPK instruments |
|---|---|
| Model | nanowizard afm |
| Category | Laboratory Equipment |
| Language | English |
Overview of the handbook's scope, content, and target audience for AFM users.
Explains AFM's fundamental principles, contrasting it with optical microscopy.
Details the physical components, shapes, and materials of AFM cantilevers.
Explains feedback loops and height adjustments for image acquisition.
Details contact and intermittent contact modes of AFM operation.
Describes phase imaging and its utility in distinguishing material properties.
Introduces force spectroscopy as a method for sensitive force measurements.
Explains how to process and plot force-distance curve data for analysis.
Covers the physical components, shapes, and materials of AFM cantilevers.
Discusses selecting cantilevers suitable for specific AFM imaging modes.
Details methods for calibrating the spring constant of AFM cantilevers.
Covers substrate choice, cell attachment, and fixation techniques.
Discusses key parameters like cantilever, setpoint, and scan rate for cell imaging.
Explains using the oscilloscope to optimize imaging parameters for cells.
Emphasizes cleanliness and substrate choice for high-resolution imaging.
Provides tips for imaging single molecules in liquid using intermittent contact mode.
Explains how tip geometry causes artifacts and affects image quality.
Describes artifacts resulting from double tips or worn/damaged probes.
Covers interaction forces, tip shape, and sample considerations.
Explains cantilever resonance behavior and the significance of the Q-factor.
Details the thermal noise analysis method for spring constant calibration.
Describes methods for cleaning AFM cantilevers and tips for optimal performance.
Explains surface modification techniques using silanes for substrates and tips.
Provides instructions for creating custom gel packs for storing AFM cantilevers.
Lists general AFM papers for further reading on cell imaging and interactions.
Lists key publications for methods of spring constant calibration.
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