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Life technologies EVOS FL - User Manual

Life technologies EVOS FL
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USER GUIDE
EVOS
®
FL
EVOS
®
FL Color
Imaging Systems for Fluorescence
and TransmittedLight Applications
Catalog Numbers AMF4300, AMEFC4300
Publication Number MAN0007988
Revision 1.0
For Research Use Only. Not for use in diagnostic procedures.

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Questions and Answers

  • S
    Steven VillarrealAug 20, 2025
    Why is the LCD screen red on my Life technologies EVOS FL All in One Printer?
    • S
      Shannon HerreraAug 20, 2025
      If the LCD screen of your Life technologies All in One Printer shows red or red patches, try dimming the illumination until the red highlights disappear. This will help you achieve the maximum brightness level without overexposing any areas. Alternatively, you can disable the 'Highlight saturated pixels in red' option located in the Settings: Basic tab.
  • M
    Marc JacksonAug 22, 2025
    What to do if image does not respond to focus on Life technologies All in One Printer?
    • A
      anthonybryanAug 22, 2025
      If the image on your Life technologies All in One Printer does not respond to changes in focus or stage position, click the LIGHT ON button. Note that if a red USB icon is visible on the Save button, it indicates there is an unsaved image. Ensure you save the image before clicking LIGHT ON to avoid losing it.
  • P
    Paul RubioAug 25, 2025
    How to fix a black LCD screen on Life technologies EVOS FL?
    • W
      William MilesAug 25, 2025
      If the LCD screen on your Life technologies All in One Printer is black, try the following: * Click the LIGHT ON button on the screen. * Ensure the objective selection wheel is positioned correctly to allow light to pass through the objective. * Check that the phase annuli selector on the condenser is not stuck between settings. * Center the specimen over the objective. * Confirm that the power supply is properly connected and the power switch is turned on.
  • W
    William BrooksAug 26, 2025
    Why DVI output does not work on Life technologies EVOS FL All in One Printer?
    • J
      jamesmcphersonAug 26, 2025
      If the DVI output on your Life technologies All in One Printer is not working with an external LCD monitor or projector: * Power off the instrument, check the cable connections, and then power the instrument back on. * Ensure that the external display accepts DVI-D input (digital input). Monitors with only analog input (DVI-A) will not display the digital output.
  • W
    wdunnAug 27, 2025
    Why transmitted light image is too dim on Life technologies EVOS FL?
    • K
      Kimberly GonzalezAug 28, 2025
      If the transmitted light image appears too dim at higher magnifications on your Life technologies All in One Printer, ensure the phase annuli selector is set to the BF position. Also, remove any condenser slider or light shield box that may be in place.
  • S
    shieldsnicholeAug 29, 2025
    What to do if LIGHT ON/OFF button is inactive on Life technologies EVOS FL?
    • S
      smithcharlesAug 29, 2025
      If the LIGHT ON/OFF button on your Life technologies All in One Printer is inactive, verify that the light cube selection lever and the objective selection wheel are each clicked into a definite position.
  • S
    suttonjamesAug 31, 2025
    How to fix mechanical stage if it does not move on Life technologies EVOS FL All in One Printer?
    • K
      Kent AllenAug 31, 2025
      If the mechanical stage of your Life technologies All in One Printer does not move, first remove the stage lock pin. Then, check the stage brakes (located on the stage knobs) and loosen them as needed.
  • S
    Sandra RobbinsSep 2, 2025
    Trouble focusing on coverslipped specimen on Life technologies All in One Printer?
    • T
      tbruceSep 2, 2025
      If you are having trouble focusing on a coverslipped specimen on a standard slide with your Life technologies All in One Printer, place the slide so the coverslip is facing up. Long working-distance objectives require a thick optical substrate and image best through 1.0 to 1.5 mm of glass or plastic.
  • P
    pharrisonSep 4, 2025
    How to fix uneven focus across screen on Life technologies EVOS FL All in One Printer?
    • L
      lori67Sep 4, 2025
      To resolve an uneven focus across the screen on your Life technologies All in One Printer, ensure that the specimen is positioned flat on the stage and that the specimen’s thickness is even.
  • C
    Christy MartinezSep 6, 2025
    Why save button does not respond on Life technologies EVOS FL?
    • K
      kristen27Sep 6, 2025
      If the save button does not respond when clicked on your Life technologies All in One Printer, ensure that you capture an image first. You can only save an image that has been captured. The USB icon on the Save button will appear red when there is a captured image ready to be saved.

Summary

Quick Reference Diagrams

Hardware Controls

Diagrams and identification of physical controls on the EVOS® FL microscope.

Hardware Glossary

Definitions for key hardware components and their functions.

User Interface (UI) Controls

Explains the software-driven controls and interface elements of the EVOS® FL system.

Software Glossary

Definitions for software terms and features used in the EVOS® FL system.

Specifications

Installing the EVOS FL Imaging System

Standard Items Included

Lists all accessories and components provided with the EVOS® FL Imaging System package.

Unpacking

Step-by-step instructions for safely removing the EVOS® FL Imaging System from its packaging.

Operating Environment

Specifies the recommended temperature, humidity, and space requirements for system setup.

Pins;Locks and Power Supply

Details on removing stage lock pins, handling light cubes, and connecting the power supply.

Ports and Shields

Information on connecting USB/DVI ports and installing protective shields for the instrument.

Basic Operations

Brightfield;Phase Operations

Guide to performing imaging using brightfield and phase contrast illumination methods.

Fluorescence Operations

Procedures for acquiring images using fluorescence channels and light cubes.

Advanced Operations

Transfection

Specific workflow for performing transfection experiments using the EVOS® FL system.

Time Lapse

Instructions for setting up and running time-lapse imaging experiments.

Working with Images

Live;Captured Zoom and Scalebar Option

How to use zoom features and add scalebars to live and captured images.

Cell Counting

Detailed steps for using the cell counting tool to quantify cells in images.

Saving

Instructions for saving captured images, including file formats and destinations.

Image Review

How to review, rename, or delete previously captured images and video files.

Optional Settings

Date;Time Settings

How to configure the system's date, time, and time zone for accurate data logging.

Network Settings

Steps to connect the EVOS® FL Imaging System to a network for image saving.

Login Settings

Managing user profiles and login preferences for the EVOS® FL system.

Maintaining the EVOS FL Imaging System

General Care

Guidelines for routine cleaning and upkeep of the EVOS® FL Imaging System.

Sterilization

Procedures for cleaning and sterilizing the instrument components.

Updating Software

Instructions for downloading and installing software updates for the EVOS® FL system.

Changing LED Light Cubes

Step-by-step guide on how to replace or install LED light cubes.

Assign Custom Channel Display Colors

How to customize the display colors for different fluorescence channels.

Appendix A: Troubleshooting

Image Quality Issues

Common problems related to image quality and their potential solutions.

Software Interface Issues

Solutions for problems encountered with the EVOS® FL software interface.

Mechanical Issues

Troubleshooting steps for mechanical problems with the EVOS® FL instrument.

Appendix B: Safety

Safety Conventions Used in this Document

Explanation of safety alert words and symbols used throughout the manual.

Symbols on Instruments

Descriptions of electrical and environmental symbols found on Life Technologies instruments.

Safety Labels on Instruments

Details on hazard symbols and associated safety warnings in English and French.

General Instrument Safety

General safety precautions for operating, moving, and cleaning the instrument.

Chemical Safety

Guidelines and warnings for handling chemicals and minimizing exposure risks.

Chemical Waste Safety

Procedures for safely handling and disposing of chemical waste generated by the instrument.

Electrical Safety

Warnings and precautions related to electrical hazards and proper power connection.

Physical Hazard Safety

Safety information regarding moving parts and potential crushing or cutting hazards.

Biological Hazard Safety

Precautions and guidelines for handling biological samples and biohazards.

Safety and Electromagnetic Compatibility (EMC) Standards

Information on compliance with safety and EMC standards for various regions.

Documentation and Support

Information on obtaining technical support, safety data sheets, and product warranty.

Life technologies EVOS FL Specifications

General IconGeneral
BrandLife technologies
ModelEVOS FL
CategoryAll in One Printer
LanguageEnglish