Life technologies EVOS FL - Appendix A: Troubleshooting; Image Quality Issues

40 EVOS
®
FL and EVOS
®
FL Color User Guide
Appendix A: Troubleshooting
Note: For additional technical support, contact your local EVOS
®
distributor. If you do not have
your distributor information, contact Technical Support (page 54).
Image Quality Issues
Problem Possible Solutions
Misaligned overlay image
• Re-capture images in each channel.
Transmitted light image is too dim (at
higher magnifications)
• Set the phase annuli selector to the BF position.
• Remove condenser slider, if one is in place.
• Remove light shield box, if it is in place.
Uneven focus across screen
• Position specimen flat on the stage; be sure the specimen’s
thickness is even.
Trouble focusing on coverslipped
specimen on standard slide
• Place the slide so the coverslip is facing up (long working-
distance objectives require a thick optical substrate, and image
best through 1.0 to 1.5 mm of glass or plastic).
LCD screen is black
• Click the LIGHT ON button (onscreen).
• Move objective selection wheel so that light shines through
objective.
• Verify that the phase annuli selector on condenser is not stuck
between settings.
• Center specimen over objective.
• Verify power supply is connected and power switch is on.
LCD screen is red, or red patches
cover parts of the screen
• Dim the illumination until the red highlights disappear to get
the maximum level of brightness without any overexposed
areas.
• Disable the “Highlight saturated pixels in red” option in the
Settings: Basic tab.