EVOS
®
FL and EVOS
®
FL Color User Guide
3
Contents
About this Guide ............................................................................................................................................. 5
1. Quick Reference Diagrams ...................................................................................... 6
Hardware Controls ......................................................................................................................................... 6
Hardware Glossary ......................................................................................................................................... 7
User Interface (UI) Controls ........................................................................................................................... 8
Software Glossary ............................................................................................................................................ 9
Specifications .................................................................................................................................................. 11
2. Installing the EVOS
®
FL Imaging System ............................................................... 12
Standard Items Included .............................................................................................................................. 12
Unpacking ...................................................................................................................................................... 13
Operating Environment ................................................................................................................................ 14
Pins/Locks and Power Supply .................................................................................................................... 15
Ports and Shields ........................................................................................................................................... 16
3. Basic Operations ................................................................................................... 17
Brightfield/Phase Operations...................................................................................................................... 17
Fluorescence Operations .............................................................................................................................. 19
4. Advanced Operations............................................................................................. 20
Transfection .................................................................................................................................................... 20
Time Lapse ..................................................................................................................................................... 21
5. Working with Images ............................................................................................. 23
Live/Captured Zoom and Scalebar Option ............................................................................................... 23
Cell Counting ................................................................................................................................................. 24
Saving .............................................................................................................................................................. 26
Image Review ................................................................................................................................................. 29
6. Optional Settings ................................................................................................... 30
Date/Time Settings ....................................................................................................................................... 30
Network Settings ........................................................................................................................................... 31
Login Settings ................................................................................................................................................ 34
7. Maintaining the EVOS
®
FL Imaging System ........................................................... 35
General Care ................................................................................................................................................... 35
Sterilization .................................................................................................................................................... 35
Updating Software ........................................................................................................................................ 36
Changing LED Light Cubes ......................................................................................................................... 37