Lonza 4D-Nucleofector Core Unit - General Instructions for Running a Nucleofection Experiment; Overview

12 Bioscience Solutions – 4D-Nucleofector™ Manual
Select Functional Unit
(chapter 2.8.2)
Select Vessel Type
(chapter 2.8.2)
Dene New Experiment
(chapters 2.9, 2.10 or 2.11)
Load Predened Experiment
(chapter 2.8.4)
Saved User ExperimentLonza Template
ModifyUse As Is
Load Samples
(chapter 2.9, 2.10 or 2.11)
Execute Nucleofection Experiment
(chapter 2.9, 2.10 or 2.11)
Prepare Samples According to Cell-type Specic Optimized Protocol
2.8 General Instructions for
Running a Nucleofection
Experiment
This chapter explains some general features and steps required to perform
a Nucleofection experiment independent of the dierent 4D-Nucleofector™
Units. The detailed handling of each unit is described in subsequent
chapters 2.9 – 2.11.
2.8.1 Overview