Lonza 4D-Nucleofector Core Unit - Running the Experiment

4D-Nucleofector™ Manual – Bioscience Solutions 17
2.9.3 Running the Experiment
10. After loading the samples press “START” to run the experiment (gure
2.15, A).
11. The progress of the experiment is indicated by changing the color
of the cuvette or well positions (gure 2.15, B) (for color codes see
chapter 4).
NOTE: When working with the single 100 µL Nucleocuvette™ Vessels you
can process two cuvettes at once. If more than two cuvettes have
been dened the drawer opens automatically after each set of
two and a message “Please change cuvette(s). Proceed?” will be
displayed (gure 2.15, C). Load next samples and press “YES” to
continue or press “NO” to interrupt the experiment.
12. When the experiment is complete, a result le summarizing the
Nucleofection process will be displayed (gure 2.15, B).
13. The result le will be saved automatically by the system and can be
reopened as described in chapter 2.12.2.
14. You can repeat the same experiment by pressing “START” again.
A message will appear asking “Do you want to pulse the same
experiment again?” (gure 2.15, D).
15. Press “OK” to start the experiment, “NEW” to return to the “Experiment”
screen and dene a new experiment or “CANCEL” to return to the
“Results” screen.
A
C
B
D
Figure 2.15: Experiment progress (X Unit)
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