Lonza 4D-Nucleofector Core Unit - Synchronize; Cleaning the Nucleofection Vessel Tray; (X or y Unit); Lonza Programs

30 Bioscience Solutions – 4D-Nucleofector™ Manual
2.12.6 Lonza Programs
The “Cell Type Program” list can be exchanged independently from a
rmware update (see chapter 2.12.7). For uploading a new “Cell Type
Program” list follow instructions below:
– Open the “Settings” menu.
– Press “LONZA Programs” (gure 2.27, K).
– Insert a USB stick comprising the le you want to upload (*.pd)
– Press “Load” and browse for le location.
– Double-click on USB folder and select new le.
– Press “OK” for upload.
2.12.7 Firmware Update
Due to technical improvement of the operation software, the
4D-Nucleofector™ System may require an update of its rmware. To update
the rmware proceed as follows:
– Start the 4D-Nucleofector™ System.
– The “Choose a Device” main screen will appear.
– Click on the Core Unit icon.
– Select “Firmware Update” within the “Settings” menu.
– A dialog box will appear asking to plug in the USB stick.
– Plug in the USB stick containing the new rmware (as provided by
Lonza) and press UPD” (gure 2.27, L).
– Follow the instructions on the screen to update the rmware.
NOTE: More comprehensive rmware updates may not be possible via
USB upload and require return of the system to Lonza.
2.12.4 Synchronize
This menu is used to synchronize the rmware of your 4D-Nucleofector™
System with the PC Editor software (see chapter 2.13). It is necessary to
perform synchronization with each rmware update.
To start synchronization:
– Insert a USB stick into the USB port on the front of the Core Unit.
– Press “Synchronize” (gure 2.17, G).
– The 4D-Nucleofector™ System is now creating a synchronization le
and saves it to the USB stick (gure 2.27, H).
– Remove the USB stick and connect it to the PC your PC Editor software
is installed.
– Open the PC Editor software as described in chapter 2.13
– Select the menu Settings and press “Synchronize”.
– A window will open enabling you to select the USB stick.
– Select the USB stick as storage device and press “OK”.
– Conrm that you’d like to update the PC Editor by pressing “YES” or
cancel by pressing “NO”.
2.12.5 Cleaning the Nucleofection
Vessel Tray (X or Y Unit)
To allow convenient cleaning, the Nucleofection vessel tray can be
removed from the X and the Y Unit. To dismount the tray please follow
the instructions below:
– Open the “Settings” menu
– Press “Device Cleaning”.
– The drawer will open, giving access to the Nucleofection vessel tray.
– Remove the tray from its support by pressing the two plastic noses
on the left and right side of the tray.
– Wash the tray with a cloth damped in water and rinse it with tap
water until all leftovers are washed o.
– Dry the tray carefully.
– Remount the dried tray to the support.
– Press “DONE”.
– The drawer will close and the tray will be moved to its home
position.