Lonza 4D-Nucleofector Core Unit - Nucleofector System Components 8 5 Purchaser Notification

8 Bioscience Solutions – 4D-Nucleofector™ Manual
2.5 4D-Nucleofector™ System
Components
By denition the 4D-Nucleofector™ System is a modular system oering
maximum exibility. Therefore, a complete system may vary in the number
and type of components it contains. Furthermore, a system may consist
of minor model variants of the units. These variants can be discriminated
by their part codes and serial numbers (table 2.1).
Table 2.1: Overview of model variants
Model Variant A Variant B
Years
2010 – 2013 2013 – Now
Part codes
Core Unit: AAF-1001B
X Unit: AAF-1001X
Y Unit: AAF-1001Y
Core Unit: AAF-1002B
X Unit: AAF-1002X
Y Unit: AAF-1002Y
LV Unit: AAF-1002L
Serial numbers
Core Unit: 5**B****
X Unit: 5**X****
Y Unit: 5**Y****
Core Unit: 8**B****
X Unit: 8**X****
Y Unit: 8**Y****
LV Unit: 9**LV***
Unit arrangement
horizontal or vertical
(via demountable side panels)
vertical only
(xed side panels)
Core Unit – display
frame
silver blue
X/Y Unit – sample rescue
(also see 3.2)
using wheel accessible after
removal of side panel
via screw behind termination
plug
A typical 4D-Nucleofector™ System includes:
1. One 4D-Nucleofector™ Core Unit
2. At least one functional unit
– X Unit: comprising two retainers for 100 μL Nucleocuvette™ Vessels
and one retainer for 16-well Nucleocuvette™ Strips
– Y Unit: enabling transfection of cells in adherence using a 24-well
Dipping Electrode Array
– LV Unit: for large volume transfection of up to 10
9
cells
3. Power cord
4. Interface cable for high voltage and data connection (plus termination
plug cap, not shown)
Figure 2.1: 4D-Nucleofector™ System before assembly
1
2
3
4
5. Touch screen (graphical user interface of the system)
6. Operation LED
7. USB port (for USB stick)
8. X Unit with tray holding retainers for two 100 μL Nucleocuvette™
Vessel and one 16-well Nucleocuvette™ Strip
9. Y Unit with tray holding 24-well Dipping Electrode Array for adherent
transfection
10. LV Unit with slot for 1 mL or LV Nucleocuvette™ Cartridge
Figure 2.2: 4D-Nucleofector™ System comprising Core, X and Y Unit (A) or Core, X and
LV Unit (B)
8
6
7
5
9
10
A
B