Lonza 4D-Nucleofector Core Unit - Using the 1 Ml Nucleocuvette Cartridge; (Fixed Volume); Defining a New Experiment; Loading Samples

4D-Nucleofector™ Manual – Bioscience Solutions 21
2.11.1 Using the 1 mL Nucleocuvette™ Cartridge
(Fixed Volume)
2.11.1.1 Dening a New Experiment
1. After unit and vessel type selection (see chapter 2.8.2) a screen
appears which allows you to dene experiment parameters select a
previously saved experiment by pressing “Choose existing experiment”
(gure 2.20, A).
2. Press the field “CELL TYPE PROGRAM” to choose predefined
Nucleofection conditions from a cell type list (gure 2.20, B). Use
the search (magnifying glass symbol) or the sort list functions (A-Z)
to nd conditions more quickly.
a. Select the desired cell type code by tapping on the appropriate
line of the cell list. The cell type selected will be highlighted. For
additional information about the cell type selected press “i”.
b. To conrm your selection press “OK”.
c. If required, modify pulse code by pressing the letter or number
code elds. A keyboard will appear, enabling you to change
settings. The solution code can be modied via a selection list.
3. Instead of dening solution and program code via the CELL TYPE
PROGRAM, both parameters can also be selected manually, e.g. in
case no predened cell type program is available. For adding new cell
type programs, please refer to chapter 2.12.3.
4. Optional: At this stage (or at step 8) you can save your dened
experiment for future use by pressing the “SAVE” button
(gure 2.20, C). A keyboard will appear allowing you to dene a name
(max. length: 26 characters).
5. You may enter further information about your experiment by touching
the “Info” eld and typing in your text (gure 2.20, D).
6. Conrm and save the experiment parameters by pressing “OK” or
“SAVE”.
7. Press OK to continue (gure 2.20, E)
8. A summary of the defined settings will be displayed (figure
2.20, F). Please check for correctness before continuing.
2.11.1.2 Loading Samples
9. Fill the 1 mL Nucleocuvette™ Cartridge with the cell-substrate solution
(gure 2.21, A ). A volume of 1 mL is required to completely ll the
cuvette. Using a higher or lower volume may lead to errors.
10. Insert the lled cartridge into the slot until it snaps in (gure 2.21, B).
C D
E F
A B
Figure 2.20: Experiment denition (LV Unit, 1 mL Nucleocu-
vette™ Cartridge)
Figure 2.21: Loading of sample (LV Unit, 1 mL Nucleocuvette™
Cartridge)
2