What to do if Lonza 4D-Nucleofector system is unable to start?
If your Lonza Laboratory Equipment system is unable to start, it's possible that the buffer battery is too low. If the device is not used for longer periods, it will require occasional recharging to avoid battery discharge and ensure functionality.
What to do if I reuse Nucleocuvettes Vessels with Lonza 4D-Nucleofector Laboratory Equipment?
It is strongly recommended to use the Nucleofection® Vessels only once with Lonza Laboratory Equipment, because the high voltage pulses that are applied drastically affect their physical integrity.
How to improve DNA quality for Lonza Laboratory Equipment?
To improve DNA quality for Lonza Laboratory Equipment, ensure that the DNA used for Nucleofection® Experiments is of high purity. It is strongly recommended to use endotoxin-free preparation of the DNA. Avoid procedures involving phenol/chloroform treatment.
What if DNA amount is too low for Lonza 4D-Nucleofector?
If both, transfer efficiency and cell mortality are low, the DNA amount could be increased when using Lonza Laboratory Equipment.
What happens if cell number is too high or too low in Lonza Laboratory Equipment?
Please use the cell numbers recommended in the dedicated optimized protocol of Lonza Laboratory Equipment.
How to avoid buffer battery discharge in Lonza Laboratory Equipment?
If Lonza Laboratory Equipment is not used for longer periods, it will require occasional recharging to avoid battery discharge and ensure functionality.
Why am I getting suboptimal transfection results with my Lonza 4D-Nucleofector Laboratory Equipment?
Suboptimal transfection results with Lonza Laboratory Equipment can stem from several factors. Your cell culture conditions might be suboptimal; ensure cells are viable and have been cultured for several passages, and avoid excessive cell densities or confluencies, which can decrease cell viability after the Nucleofection® Procedure. Poor DNA quality can also cause issues, so use high-purity, endotoxin-free DNA and avoid phenol/chloroform treatments. The DNA amount might be too low; increase it if both transfer efficiency and cell mortality are low. Ensure you are using the cell numbers recommended in the dedicated optimized protocol, as having too high or too low a cell number in the Nucleofection® Sample can cause problems. Finally, if the device isn't used for extended periods, occasion...
Explains the core components and principles of Nucleofector® Technology.
Highlights broad applicability for cells, substrates, and optimized protocols.
Describes the system's development and key features like flexibility and scalability.
Covers usage limitations, maintenance, and critical safety warnings.
Describes the central unit of the modular 4D-Nucleofector® System.
Lists the available functional units (X, Y, 96-well, LV) connecting to the Core Unit.
Explains powering on/off and navigating the touch screen software.
Outlines the general workflow, unit/vessel selection, and parameter definition.
Explains defining parameters like sample type, cell code, pulse, and solution.
Guides on loading and modifying existing or template experiments.
Explains loading samples and running/monitoring experiments on the X Unit.
Details loading samples and running/interpreting results for Y Unit experiments.
Explains loading samples and running/interpreting results for 96-well unit experiments.
Lists the parts of the 1 mL Nucleocuvette® Cartridge.
Lists the parts of the LV Nucleocuvette® Cartridge and associated reservoir.
Lists components of the LV Nucleocuvette® Cartridge with weldable tubing.
Explains loading samples and running/interpreting results for the 1 mL LV Unit.
Guides on setting up a new experiment for the scalable LV Unit, including volume and bubble detection settings.
Details the steps for mounting the LV Nucleocuvette® Cartridge.
Explains how to load samples into the scalable LV Unit cartridge.
Guides on running, monitoring, and interpreting results for the scalable LV Unit.
Accesses and details system settings like time, date, brightness, and network.
Covers cleaning procedures and checking unit software/firmware versions.
Manages pre-installed templates and user-saved experiments, and results.
Explains creating and editing custom cell type codes for experiments.
Instructions on installing the PC Editor and transferring experiments.
Guides on exporting, viewing, and printing result files using the PC Editor.
Provides solutions for common issues like low survival rate and low efficiency.
Describes how to rescue samples during system failure or power outage.
Explains color coding for well status and the LV Unit rating system.
Explains arc discharge phenomena and system response.
Lists and explains various error codes encountered with the system.
Details the license terms and usage restrictions for the system.
Outlines Lonza's warranties and limitations of liability.
General| Technology | Nucleofection technology |
|---|---|
| Applications | Cell transfection, gene editing, cell reprogramming |
| Compatibility | Compatible with a wide range of cell types |
| User Interface | Touchscreen |
| Data Management | USB export |
| Throughput | Capability to process single samples or 96-well plates |
| Sample Volume | 20 µL to 100 µL |
| Cell Types | Primary cells, stem cells, and cell lines |
| Programming | Pre-set and customizable protocols |
| Power Requirements | 100-240 V AC, 50-60 Hz |
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