Lonza 4D-Nucleofector - System Error Code Reference

4D-Nucleofector
®
Manual | 39
4
4.4 4D-Nucleofector
®
System Error Codes
The 4D-Nucleofector
®
System detects and repots different
types of errors. Some are just error messages, others might
be indicated by a code.
The table below explains the result codes (errors or results)
that can be indicated in the next generation 4D-Nucleofec-
tor
®
System. For most codes this information is also shown
in the software. This list does not contain LV Unit specific
error codes yet
Model Code X Unit Y Unit 96-well Unit LV Unit What Happened? Possible Error Procedure
Err 1A x x x x No pulse generated Internal communica-
tion failure, internal
error or device possibly
defective
Clear the error message by pressing any but-
ton and try to apply program a second time. If
Err1A re-occurs, switch off the device, check
the interface cable, wait for 2 seconds and
switch on again. If error persists, contact our
Scientific Suppot
Err 2 x
x x
x
No pulse generated No or inappropriate
cuvette loaded. Inap-
propriate Nucleofector
®
Solution or volume
Check cuvette. Check type and volume
of Nucleofector
®
Solution. Cuvette can be
reloaded.
Err 3 x x x x No pulse generated Improper interconnec-
tion or system defective.
Switch off the device. Check proper connec-
tion of the interface cable as well as the termi-
nator cap on the last interface outlet. Switch
on the device and repeat the experiment with
the same sample.
Err 4 -WEAK x x x x A weak pulse occurred.
Efficiency may be sub-
optimal.
Re-used cuvette or inap-
propriate Nucleofector
®
Solution
Clear the error message by pressing any but-
ton. Use the sample if maximum efficiency is
not essential. Otherwise, use a new sample.
Err 7 x x x - No pulse generated No lid detected on the
cuvette or plate
Clear the error message by pressing any but-
ton. Check proper placement of the lid and
rerun experiment with the same cuvette
Err 8 x x x x Device possibly generated
an arc discharge leading to
incomplete program execu-
tion.
A substantial reduction in
efficiency and viability must
be assumed
Inappropriate cuvette,
Nucleofector® Solu-
tion or volume in the
cuvette.
Clear the error message by pressing any
button. Check the volume of Nucleofector
®
Solution. Try again with a new cuvette.
Err 11 x x - - No pulse generated.
The device case is
open.
Switch the device off, check the cor-
rect mounting of all side panels and
the interlock between all modules. Try
again with the same samples. Can only
occur with model variants A of first
generation.
Err 12 x x x - Pulse possibly omitted. No
pulse generated.
Internal device tem-
perature too high for
pulsing.
Switch off the device, wait for 2 min-
utes and run experiment with a new
cuvette.
Err 13 x x x - No pulse generated.
The device front flap
is open.
Check proper closing of front flap after
the tray is closed and rerun the experi-
ment.
Err 21
Used well
- x - - Used well:
Multiple use of well. Nucleo-
fection® Process may be
suboptimal.
Dipping electrode
for that well position
was used twice.
Utilize sample if maximum performance
is not essential. Avoid re-use of dipping
electrodes that had been used before.
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