Lonza 4D-Nucleofector - LV Unit Consumables; 1 mL Nucleocuvette Cartridge Components; LV Nucleocuvette Cartridge and Reservoir Components; Weldable Tubing Cartridge Components

22 | 4D-Nucleofector
®
Manual
2.12 4D-Nucleofector
®
LV Unit
The 4D-Nucleofector
®
LV Unit can handle two types of
vessels, the fixed volume 1 mL Nucleocuvette
®
Catridge for
cell numbers up to 1×10
8
cells and the scalable flow-through
LV Nucleocuvette
®
Catridge for cell numbers up to
1×10
9
cells.
Components of 1 mL Nucleocuvette
®
Catridge
(Figure 2.31, A)
1. Transparent handle/handle bar
2. Contacting side/pat/area
3. Injection pot (rear side)
4. Air outlet pot with 0.2 M sterile filter
Components of the LV Nucleocuvette
®
Catridge
(Figure 2.31, B, C)
1. Catridge
2. Venting tube with 0.2 M sterile filter
3. Inlet pot 1 (spinning Spiros with red dust protection cap)
4. Inlet pot 2 (spinning Spiros with red dust protection cap)
5. Outlet pot (spinning Spiros with red dust protection cap)
6. Reservoirs (swabbable injection pot with male Luer cap)
NOTE: Please do not alter or disassemble the pre-assembled
catridge or reservoirs.
Components of the 4D-Nucleofector
®
LV Reservoir
(Figure 2.31, C)
1. Reservoir cap
2. Reservoir body
3. Outlet tube (weldable)
4. Outlet pot (swabbable injection pot (with male Luer cap)
Components of the LV Nucleocuvette
®
Catridge, weldable
tubing (Figure 2.31, D)
1. Catridge
2. Venting tube with 0.2 M sterile filter
3. Inlet pot 1 (spinning Spiros with red dust protection cap)
4. Inlet pot 2 (spinning Spiros with red dust protection cap)
5. Outlet pot (spinning Spiros with red dust protection cap)
6. PVC tube attachments with clamps (for welding)
Figure 2.31: 4D-Nucleofector
®
LV Unit Consumables
2
1
4
3
A
5
4
3
1
2
B
2
1
3
4
C
5
4
3
1
2
D
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