Lonza 4D-Nucleofector - Operating Instructions and Safety; Restrictions, Maintenance, and Safety Precautions

6 | 4D-Nucleofector
®
Manual
2.1 Restrictions
Medical use restrictions
Nucleofector
®
Technology is intended for research and
investigational use by professionals only. Please note that
Lonza’s Nucleofector
®
Technology is not intended to be
used for diagnostic purposes, nor for testing or treatment
in humans.
License statement
Lonza is holder of various patents, patent applications, cop-
yrights and technical and scientific experience with respect
to Nucleofector
®
Technology. Use of Lonza’s Nucleofector
®
Technology and /or related software requires a license from
Lonza.
Purchasers are granted a non-exclusive, non-transferable
license only for a limited use of Lonza’s Nucleofector
®
Technology and related software for research and develop-
ment purposes, the terms of which are disclosed in detail in
chapter 5. Cetain commercial application is allowed under
Lonza’s license for for-profit-entities, as set foth in the
license terms with any such entities.
For license information contact Lonza Cologne GmbH:
Phone: + 32 87 321 611
E-mail: ip.cologne@lonza.com
2.2 Maintenance
The 4D-Nucleofector
®
System requires minimal mainte-
nance for reliable operation. Unplug the power supply
before cleaning and disinfecting the case. Use a damp cloth
to wipe down the outer case with water or 70–80% etha-
nol. Do not use any aerosols for cleaning. Avoid wetting the
cuvette holder within the cuvette tray and the connectors
located on the rear of the system.
The 4D-Nucleofector
®
System is designed for use under a
sterile hood either with or without a UV radiation source.
Prolonged exposure of the outer casing to UV light may
lead to discoloration but not functional impairment of the
Nucleofector
®
System. However, the system should be re-
moved from the hood during extended UV irradiation.
The 4D-Nucleofector
®
System is protected by two main
fuses. Both are inside a receptacle incorporated in the
power socket of the Core Unit (see figure 2.1). Blown fuses
can easily be replaced. Disconnect the 4D-Nucleofector
®
System from the power supply and inset a small flat screw-
driver into the slot on top of the power plug housing to lever
it open. The fuse receptacles (each marked with an arrow)
are then also levered open using the screwdriver.
Figure 2.1: Opened fuse receptacles
The 4D-Nucleofector
®
System will not function unless both
the left and right receptacle contain intact fuses. Blown
fuses can usually be identified by molten broken wires
inside the glass tube.
Only use T2.5A, L fuses to replace blown fuses.
2.3 Safety instructions – please read carefully
This symbol means that there is a risk of electric
shock. An electric shock could cause death or
personal injury.
The 4D-Nucleofector
®
System has been cetified by interna-
tional safety standards and is safe to use when operated in
accordance with this manual.
This system is designed to deliver variable high voltage elec-
trical impulses for the purpose of introducing substrates
into eukaryotic cells.
These electrical impulses can be deadly!
2 Operating Instructions
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