Lonza 4D-Nucleofector - System Components Identification; 4 D-Nucleofector Core Unit; Functional Unit Types

4D-Nucleofector
®
Manual | 9
1. Touch screen (graphical user interface of the system)
2. Power Switch LED
3. USB pots (for USB sticks)
Figure 2.3: (A) 4D-Nucleofector
®
Core Unit, (B) 4D-Nucleofector
®
System
comprising of Core, X, and 96-well Unit in rear view.
4. Power cord socket with main power switch and fuses
5. Power cord
6. USB pot(s)
7. Lonza interface outlet pot to connect functional units
8. Lonza interface inlet pot to connect functional units
9. Lonza interface inlet pot of last functional unit
10. Lonza interface cable connected to outlet and inlet pot
11. Lonza interface outlet pot of functional unit
12. Lonza outlet pot of last functional unit with termination
plug
1
2
3
4
7
11
8
12
6
5
10
9
2
2.5 4D-Nucleofector
®
System components
By definition, the 4D-Nucleofector
®
System is a modular
system offering maximum flexibility. Therefore, the number
of units in a system might vary, as one Nucleofector
®
Core
Unit can operate up to three functional units. The units may
also belong to different 4D Nucleofector
®
System variants.
These variants can be distinguished by their pat codes and
serial numbers (Table 2.1).
This manual only describes the use of variant C. For older
variants A or B please refer to the manual of the first gen-
eration 4D-Nucleofector
®
System.
Table 2.1: Overview of model variants
Model Variant A Variant B Variant C
Years 2010 – 2013 2013 – 2020 2021 - now
Pat codes Core Unit:
AAF-1001B
X Unit: AAF-1001X
Y Unit: AAF-1001Y
Core Unit:
AAF-1002B
X Unit: AAF-1002X
Y Unit: AAF-1002Y
LV Unit: AAF-1002
Core Unit: AAF-1003B
X Unit: AAF-1003X
Y Unit: AAF-1003Y
LV Unit: AAF-1002LW
96-well: AAF-1003S
Serial
numbers
Core Unit:
5**B****
X Unit: 5**X****
Y Unit: 5**Y****
Core Unit: 8**B****
X Unit: 8**X****
Y Unit: 8**Y****
LV Unit: 9**LV***
Shuttle: 9**S****
Core Unit: 9**B2***
X Unit: 9**X2***
Y Unit: 9**Y2***
LV Unit: 9**LV2**
96-well: 9**S2***
Unit
arrangement
Horizontal or vetical
(via demountable
side panels)
Vetical only
(fixed side panels)
Core Unit –
display frame
Silver Blue Blue
A typical 4D-Nucleofector
®
System includes (Figure 2.2 and
Figure 2.3 A and B):
1. 4D-Nucleofector
®
Core Unit
2. At least one functional unit from the following list:
• X Unit: comprising two retainers for 100 L
Nucleocuvette
®
Vessels and one retainer for 16-well
Nucleocuvette
®
Strips
• Y Unit: enabling transfection of cells in adherence us-
ing a 24-well Dipping Electrode Array
• 96-well Unit: for medium throughput transfection of
up to 96 samples in 20 L format
• LV Unit: for large volume transfection of up to 10
9
cells
Figure 2.2: 4D-Nucleofector
®
System in different configurations. Up to
three functional Nucleofector
®
Units can be operated with on
Nucleofector
®
Core Unit.
Return to TOC