Lonza 4D-Nucleofector - Default Chapter; Table of Contents

4D-Nucleofector
®
Manual | 3
Content
1 Nucleofector
®
Technology 4
How it works 4
Transfection of any cell 4
Transfection of any substrate 4
Optimized protocols 4
Moving transfection into the
fouth dimension 5
Experience the advanced
Nucleofector
®
Platform: 5
2 Operating Instructions 6
2.1 Restrictions 6
2.2 Maintenance 6
2.3 Safety instructions – please read carefully 6
2.3.1 Consignes de sécurité - à lire attentivement 7
2.4 Waste disposal 8
2.5 4D-Nucleofector
®
System components 9
2.6 Set-up instructions 10
2.7.1 Turning on the 4D-Nucleofector
®
System 10
2.7.2 Operating software 10
2.7.3 Turning off the 4D-Nucleofector
®
System 11
2.8 General instructions for running a
Nucleofection
®
Experiment 11
2.8.1 Overview 11
2.8.2 Unit and vessel selection 11
2.8.3 Experiment parameters – overview 12
2.8.4 Using a predefined experiment 13
2.9 4D-Nucleofector® X Unit 14
2.9.1 Defining a new experiment 14
2.9.2 Loading samples 16
2.9.3 Running the experiment 16
2.10 4D-Nucleofector
®
Y Unit 17
2.10.1 Defining a new experiment 17
2.10.2 Loading samples 18
2.10.3 Running the experiment 18
2.11 4D-Nucleofector
®
96-well Unit 19
2.11.1 Defining a new experiment 19
2.11.2 Loading samples 21
2.11.3 Running the experiment 21
2.12 4D-Nucleofector
®
LV Unit 22
2.12.1 Using the 1 mL Nucleocuvette
®
Catridge (Fixed volume) 23
2.12.1.1 Defining a new experiment 23
2.12.1.2 Loading Samples 24
2.12.1.3 Running the experiment 24
2.12.2 Using the LV Nucleocuvette
®
Catridge (Scalable volume) 25
2.12.2.1 Overview 25
2.12.2.2 Defining a new experiment 26
2.12.2.3 Mounting an LV Nucleocuvette
®
Catridge 27
2.12.2.4 Loading Samples 27
2.12.2.5 Running the experiment 30
2.13 Additional settings and options 31
2.13.1 Settings menu on the right 31
2.13.1.1 System settings 31
2.13.1.2 Cleaning the Nucleofection
®
Vessel Tray (X, Y, or 96-well Unit) 32
2.13.1.3 Version 32
2.13.2 Left menu 32
2.13.2.1 E xperiments 32
2.13.2.2 Results 33
2.13.2.3 Custom Cell type codes 33
2.13.3 Lonza programs 34
2.13.4 Firmware update 34
2.13.5 Software update of functional units 34
2.14 4D-Nucleofector
®
PC Editor Software 34
2.14.1 Installation of the PC Editor 34
2.14.2 Transfer of experiments from the PC Editor 35
2.14.3 Viewing and printing result files 35
3 Troubleshooting 36
3.1 Suboptimal transfection results 36
3.2 Rescue of samples (X and Y Unit) 37
4 Error Codes of the
4D-Nucleofector
®
System 38
4.1 Color coding of wells (X, Y and 96-well Unit) 38
4.2 Result rating (LV Unit) 38
4.3 Arc discharge correction 38
4.4 4D-Nucleofector
®
System Error Codes 39
5 Purchaser Notification 41
Purchaser notification 41
Representations and warranties 41
6 Technical Specifications 43