Lonza 4D-Nucleofector - Error Codes and Results Interpretation; Understanding Well Status and LV Unit Ratings; Arc Discharge Correction Explanation

38 | 4D-Nucleofector
®
Manual
4 Error Codes of the
4D-Nucleofector
®
System
4.1 Color coding of wells (X, Y and 96-well Unit)
Well symbols and colors differ depending on the status of
the well. In the “Result” screen, the color of a well indicates
the completion status of each well (Table 4.1). In case of an
error or warning, the respective error code can be displayed
by clicking on the well in the result file.
4.2 Result rating (LV Unit)
For 4D-Nucleofector
®
LV Unit experiments no well coding
exists. Since a Nucleocuvette
®
Catridge consists of several
segments symbols are used to provide a summary rating for
the whole sample based on the percentage of segments
which were pulsed properly (OK) and segments where an
error occurred. Following thresholds were empirically de-
fined by Lonza.
4.3 Arc discharge correction
Arcing is a complete or patial discharge circumventing the
sample and is often accompanied by a flash and a noise.
This problem is usually caused by imperfect cuvettes or
cuvette filling. The 4D-Nucleofector
®
System is equipped
with a hardware safety feature that immediately detects
arc formation and protects the cells from damage. After
the arc interruption the 4D-Nucleofector
®
System re-
sumes program execution. Normally, the program can be
completed successfully (“arc discharge correction 1 or 2”)
and only minor differences in transfection efficiency are
observed. Such events are not considered as an error and
just indicated as a notes. When repeated arc discharges
occur within one program, it may be impossible for the
4D-Nucleofector
®
System to complete execution of the
program. In this case (“Err8 – Arc discharge”), significant
impact on transfection efficiency may be observed. Discard
the cuvette (which is possibly damaged) and its content,
reset the device by pressing any button and repeat the
experiment with a new cuvette. It is not necessary to switch
off the 4D-Nucleofector
®
System.
Do not re-use consumables.
Experiment Result, OK Result, Warning Result, Error Result. Skipped Well
Undened
Sample
No Program
No DNA
Table 4.1: Color code of wells
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