Lonza 4D-Nucleofector - Page 40

40 | 4D-Nucleofector
®
Manual
www.lonza.com/nucleofection-error-codes
If a problem cannot be resolved, please call
Lonza’s Scientific Suppot Team.
Europe
Phone: + 49 221 99199 400
E-mail: scientific.suppot.eu@lonza.com
Noth America
Phone: 800 521 0390 (toll free)
E-mail: scientific.suppot@lonza.com
Model Code X Unit Y Unit 96-well Unit LV Unit What Happened? Possible Error Procedure
Err 22 - x - - Used well:
A weak pulse occurred. Ef-
ficiency may be sub-optimal.
Re-used dipping elec-
trode or inappropriate
Nucleofector
®
Solution.
Clear the error message by pressing any but-
ton. Use the sample if maximum efficiency is
not essential. Otherwise, use a new sample.
Err 23 - x - - Used well:
Device possibly generated an
arc discharge. Program could
be resumed and successfully
completed. A limited impair-
ment on performance has to
be presumed.
Inappropriate solution
or well volume, oc-
casional arcing.
Utilize sample if maximum performance is
not essential. Check solution and volume.
Err 24 - x - - Used well:
Device possibly generated an
arc discharge. Program could
be resumed and successfully
completed. A limited impair-
ment on performance has to
be presumed.
Inappropriate solution
or well volume, oc-
casional arcing.
Utilize sample if maximum performance is not
essential. Check solution and volume.
Err 25
- x - - Used well:
Device possibly generated an
arc discharge leading to in-
complete program execution.
A substantial reduction in
efficiency and viability must be
assumed.
Inappropriate dipping
electrode,
Nucleofector
®
Solution
or volume in the dip-
ping electrode. Device
possibly defective.
Clear the error message by pressing any but-
ton. Check the volume of Nucleofector® Solu-
tion. Try again with a new dipping electrode.
Err 26 - x - - Used well: Multiple use of well
Nucleofection suboptimal
Dipping electrode for
that well position was
used multiple times.
Utilize sample if maximum performance is not
essential. Avoid re- use of dipping electrodes
that had been used before.
Res 27 - x - - Used well:
Well was skipped
User confirmed to skip
the well as it had been
used before
-
Return to TOC