Lonza 4D-Nucleofector Core Unit - Nucleofector LV Unit

20 Bioscience Solutions – 4D-Nucleofector™ Manual
2.11 4D-Nucleofector™ LV Unit
The 4D-Nucleofector™ LV Unit can handle two types of vessels, the xed
volume 1 mL Nucleocuvette™ Cartridge for cell numbers up to 1x10
8
cells
and the scalable LV Nucleocuvette™ Cartridge for cell numbers up to 1x10
9
cells.
Components of 1 mL Nucleocuvette™ Cartridge
(gure 2.19, A)
1. Transparent handle/handle bar
2. Contacting side/part/area
3. Injection port (rear side)
4. Air outlet port with 0.2 µM sterile lter
Components of the LV Nucleocuvette™ Cartridge
(gure 2.19, B, C)
1. Cartridge
2. Venting tube with 0.2 µM sterile lter
3. Inlet port 1 (spinning Spiros with red dust protection cap)
4. Inlet port 2 (spinning Spiros with red dust protection cap)
5. Outlet port (spinning Spiros with red dust protection cap)
6. Reservoirs (swabbable injection port with male Luer cap)
NOTE: Please do not alter or disassemble the pre-assembled cartridge or
reservoirs.
2
1
4
3
A
5
4
3
1
2
B
6
C
Figure 2.19: Nucleocuvette™ Cartridge types for LV Unit