Lonza 4D-Nucleofector Core Unit - Loading Samples; Running the Experiment

4D-Nucleofector™ Manual – Bioscience Solutions 19
2.10.2 Loading Samples
9. Insert the 24-well Dipping Electrode Array into the 24-well culture plate
containing your Nucleofection samples. Make sure that the dipping
electrode array is inserted in the right orientation.
10. Place 24-well plate with inserted dipping electrode array into the
retainer of the 4D-Nucleofector™ Y Unit. Well “A1” must be in upper
left position. If the array-plate sandwich was entered in the wrong
orientation an error message will appear after pressing “START”.
11. It is not recommended to re-use dipping electrodes as this may lead
to suboptimal transfection eciencies. An RFID chip contained in
the dipping electrode lid logs usages and after pressing “START” the
software will check for used wells and oer you dierent options how
to continue.
2.10.3 Running the Experiment
12. After loading the samples press “START” to run the experiment (gure
2.18, A).
13. The progress of the experiment is indicated by changing the color
of the cuvette or well positions (gure 2.18, B) (for color codes see
chapter 4).
14. When the experiment is complete, a result le summarizing the
Nucleofection process will be displayed (gure 2.18, C).
15. The result le will be saved automatically by the system and can be
reopened as described in chapter 2.12.2.
16. You can repeat the same experiment by pressing “START” again.
A message will appear asking “Do you want to pulse the same
experiment again?”.
17. Press “OK” to start the experiment, “NEW” to return to the “Experiment”
screen and dene a new experiment or “CANCEL” to return to the
“Results” screen.
A B
C
Figure 2.17: Loading of samples (Y Unit)
Figure 2.18: Experiment progress (Y Unit)
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