Lonza 4D-Nucleofector Core Unit - Results; Custom Programs

4D-Nucleofector™ Manual – Bioscience Solutions 29
2.12.2 Results
This menu will display a list of result les and allows you to manage
them. Result les are listed in chronological order with newest results
at the top. To open existing result les just select the le you’d like
to open and press “VIEW”. To erase result les that are no longer required,
select the le from the list and press “CLR”. To store result les on a USB
stick, insert the USB device into the USB port on the front of the Core
Unit and press “SAVE”. Pressing “i” displays further information related
to the result le (gure 2.27, D).
2.12.3 Custom Programs
The software oers the possibility to add custom program codes for
choosing a dened combination of a Nucleofector™ Program and Solution,
e.g., after an optimization. Custom program codes are included in
alphabetical order into the “Cell Type Program” list and can be dierentiated
from Lonza dened code by color:
Blue: Lonza dened cell type program codes
Black: Customer dened codes. You can create new custom programs
by pressing the “NEW” eld. Edit existing programs by pressing “EDIT” or
save your programs onto a USB stick by pressing “SAVE” (gure 2.27, E).
To create a new custom program or to edit an existing program:
– Press “NEW” or select an existing program and press “EDIT”.
– A screen will open comprising a keyboard, “PULSE CODE” setting, a
“SOLUTION” eld and a “VOLUME” selection (gure 2.27, F).
– Dene a name for your custom program by typing, e.g., the cell name.
– Press “SOLUTION” to display a list of available solutions.
– Select the solution most suited for your cell from the list and conrm
your selection with “OK”.
– Dene the Nucleofection program by typing in the “PULSE CODE” — you
can determine the best program by using one of our optimization kits
or simply use the code from an existing optimized protocol.
– Select functional units for which the code should apply (either X and
LV Unit or Y Unit).
– Conrm the setting for your custom program by pressing “OK”.
– The custom program can now be selected for an experiment by
pressing the “CELL TYPE LIST” eld.
Custom program codes won’t be overwritten in case an updated program
denition le is installed (see chapter 2.12.6).
E Menu “Custom Programs” F Menu “Custom Programs”
H Menu “ Synchronization”G Menu “ Synchronization”
Figure 2.27: Continued
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