24
D. Setting up and Running the
Instrument
Turn the instrument on by pressing the power switch on
the back of the instrument to ON.
You will need to prime the tubing cartridge whenever you
install a different cartridge on the instrument.
Note: The tubing cartridge is already calibrated when you
receive it; you do not need to calibrate it.
You can then create, load and save programs and
operate the instrument programmatically OR operate
the instrument manually. You can also adjust tip-to-well
alignment by adjusting the X-axis stage position and you
can change the dispensing speed setting.
This section describes those priming, programming,
operation and adjustment tasks.
1. Prime the Tubing Cartridge
Whenever you change the tubing cartridge, you will need
to prime it. The fi rst time you use the instrument, you will
need to prime the tubing set.
To prime the tubing cartridge:
1. Put the supply ends of the channel tubes in a
receptacle containing dH
2
O or dispense medium.
2. Ensure that the waste-fl uid vessel is below the
dispensing end of the channel tube and that the
open end of the waste-fl uid tubing is in a receptacle,
with the end of the tubing ABOVE any liquid in that
receptacle.
3. Lower the nozzle tips so that they are at the correct
dispensing height above the waste-fl uid vessel or a
plate on the stage.
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