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Tubing Cartridge Maintenance and Recalibration
2. Recalibrate the Tubing Cartridge If Necessary
Note: The tubing cartridges to be recalibrated must be
allowed to equilibrate to lab-room conditions for at least 2
hours prior to calibration.
Use a gravimetric test or optical scanning with a plate
reader to determine whether a particular channel needs to
be recalibrated.
1. Turn on the WellMate unit and set the volume to
50µl.
2. Verify that the dispense mode is set for (ten)
columns of dispense, with 1-10 ON and with
11-12 OFF.
3. Weigh a tube and tare out the scale. Use this tube to
collect the dispensed volume of each channel during
the calibration sequence. Zero the scale between
readings.
Note: There is a purge before each dispense cycle of
the WellMate. It is important NOT to collect this drop
in the tube as you perform the following operation.
Allow the drop to fall prior to putting the tube under
the nozzle.
4. Remove all tubes from the supply water, except
channel 1, and make sure that this channel is still
primed. Hold a tube under the channel 1 nozzle.
Press the START key. Proceed to collect each of
the (10) dispensed shots in the tube.
5. Weigh the water in the tube. The target weight of the
liquid is 498 mg–502 mg. If the weight is not within
these limits, use the 7/64-inch Allen wrench to turn
the adjustment screw for the channel tube (on the
supply side of the tubing).
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