39
®
E. Tubing Cartridge Maintenance
and Recalibration
This section describes how to care for the tubing cartridge
and then also how to recalibrate the cartridge if you wish to
do so.
Note: Recalibration is generally not necessary. The tubing
cartridges that come with your system have already been
calibrated. That calibration will generally last until the
precision limit for the tubing cartridge (1000 dispenses of
100µl each into a 96-well plate) has been reached. In most
cases, particularly if all of the channels in the cartridge
are no longer meeting precision specifi cations, you will
simply want to replace the disposable cartridge with a new
cartridge.
To optimize performance and extend the life of your tubing
assembly, refer to the Helpful Hints section on page 43.
1. Clean the Tubing Assembly
Table 6. Care and Maintenance of Tubing Assembly
Task How Often? Procedure
Rinse tubing After each
sample.
Use dH
2
O to rinse the tubing assembly
each time you change samples. Then
rinse the tubing with alcohol to remove
water.
Wash tubing At end of
day’s use
or between
solutions.
Use a mild detergent solution or 10%
bleach solution to clean the tubing.
Then rinse it with fresh water and fi nally
with alcohol.
Autoclave tubing
if desired
As needed.
No more
than 3x
At 120º C for 20 minutes
Replace tubing As needed. Obtain a new tube assembly cartridge
and install as you did the earlier set.
Clean nozzle tips As needed. Inspect the nozzle tips for signs of
clogging or debris. Clean them with
water and then rinse them with alcohol.
Tubing Cartridge Maintenance and Recalibration
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