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Matrix WellMate - Page 40

Matrix WellMate
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35
Setting up and running the Instrument
®
Note: Liquids tend to adhere to the walls of small tubing
(such as tube sets for 384-well plate), especially when
dispensing smaller volumes (9µl or less). To minimize the
amount of liquid retained in the tubing, make sure that the
Y-axis arm positions the tips directly over the middle of
the wells (see page 18 for Y-axis arm adjustment) and the
height gauge is used to set the nozzle over the plate.
1. Put a 96-well plate on the stage. Select plate type
96 (on the nozzle holder) and then turn off the
instrument.
2. Press the START and STOP keys simultaneously
and turn on the instrument.
3. Press START. Verify that the plate type LED on the
display and the plate selector are both set to plate
type 96.
4. Press START. The stage will move automatically so
that column 7 is under the nozzle tips.
5. Press the UP and DOWN arrow keys to adjust the
stage position until the tips are aligned in the wells
as you want them to be. (The LED display will show
a volume range from -300 to +300).
6. Press RESET, select plate type 384 (on the nozzle
holder) and verify that the 384 LED is illuminated.
7. Replace the 96-well plates with a 384-well plate
and press START. The 384-well plate will move to
column 13.
8. Press the UP and DOWN arrow keys to adjust the
stage position until the tips are aligned with the
wells.
9. Press RESET when you have nished the
adjustment and then turn off the instrument.