NOVA BIOMEDICAL BIOPROFILE FLEX2 - Page 35

5-3
5 Maintenance
5. Maintenance
5.1.2 Change ESM Tubing
The Change ESM Tubing function allows an operator with appropriate privileges to
change the ESM External Tubing Set. To prevent build-up and blockages of sample
debris and protein in the ESM ow path and to preserve the integrity of the ESM External
Tubing Set, this component should be replaced on a routine basis. The tubing set is
comprised of four individual lines of tubing—Sample (CF), Waste (EF), Cup Wash (CW)
and Performance Check (PC)—each of which is labeled with a white tag at either end.
WARNING: Cell culture samples are potential sources of infectious agents. Treat the
Sample (CF) and Waste (EF) lines as biohazardous. Gloves and protective
clothing are recommended.
NOTE: Nova Biomedical recommends that the pump tubing typically be changed every
12 months. This frequency may vary depending on system usage, and should
be replaced as needed.
To replace the ESM tubing:
1. Start the Change ESM Tubing Sequence: Select the Change ESM Tubing icon
from the ESM Maintenance screen, then select Start in the Command Bar. When
prompted, enter the Lot/Part Number of the new tubing set and press the green
checkmark to begin the sequence. The system will purge the ESM owpath of
uid. Once the green Continue button appears in the Command Bar, proceed to
Step 2.
2. Replace the Sample (CF) Tubing Line: Carefully
disconnect the Sample (CF) line from the right-hand
cover of the FLEX2 (Fig. 5.3) by turning the tting
counterclockwise until it releases. Disconnect the
opposite end of the line from the base of the ESM
sample cup (Fig. 5.5-5.6) in the same way. Discard
the old tubing and secure the new CF line to the
FLEX2 cover and sample cup by turning the ttings
clockwise until nger-tight.
NOTE: The connectors on either end of the CF line
are identical, therefore the orientation of the
CF line is arbitrary.
WARNING: Exercise caution when replacing the tubing lines
connected to the sample cup as to not alter cup
position. A signicant shift in cup position could
require realignment of the ambr® liquid handling
arm to the cup location
3. Replace the Waste (EF) Tubing Line: Carefully disconnect
the Waste (EF) line from the right-hand cover of the FLEX2
(Fig. 5.3) by turning the tting counterclockwise until it
releases. Disconnect the other end of the tubing from the
ESM syringe pump (Fig. 5.4) in the same way. Discard
the old tubing and secure the new EF line to the FLEX2
cover and syringe pump by turning the ttings clockwise
until nger-tight.
NOTE: The connectors on either end of the EF line are identical, therefore the
orientation of the EF line is arbitrary.
Figure 5.3 ESM Sample and Waste
Tubing installed on FLEX2 cover
Figure 5.4 ESM Waste, Cup
Wash, and Performance Check
Tubing connections to ESM
syringe pump
EF
CW
PC