PerkinElmer Lambda 25 User Manual

Installation
69
Minimum Volume Applications
To measure minimum sample volumes, use microcells (offered by PerkinElmer).
The minimum sample volume required is a function of the cell internal width or
volume and is specified below.
Cell Type Cell
Internal
Width
Pathlength Minimum
Volume
Required
Part
Number
2 mm 1 cm 200 µL B0631071
(pair)
Height of
liquid slightly
more than
height of
beam
4 mm 1 cm 400 µL B0631064
(pair)
Cell
Volume
Pathlength Minimum
Volume
Required
Part
Number
0.5 µL 0.01 cm 2 µL B0631082
2.5 µL 0.5 cm 5 µL B0631080
5 µL 0.1 cm 10 µL B0631083
5 µL 1.0 cm 10 µL B0631081
Cell window
completely
filled with
liquid
30 µL 1.0 cm 50 µL B0631079
NOTE: You should align microcells very carefully in the radiation beam by following the
procedures in Aligning the Single Cell Holder on page 65. When aligning
microcells, fill each cell with the minimum volume of liquid specified in the above
table to make sure that the liquid meniscus is not in the radiation beam.
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PerkinElmer Lambda 25 Specifications

General

Brand	PerkinElmer
Model	Lambda 25
Category	Laboratory Equipment
Language	English