RT-7200 Auto Hematology Analyzer User’s Manual
Parameter Reference R nge a
WBC ≤ 0.2 × 10
9
/ L
RBC ≤ 0.03 × 10
12
/ L
HGB ≤ 2 g / L
HCT
≤0.5 %
PLT ≤ 10 × 10
9
/ L
If blank test result exceeds this range, operator has to repeat above test steps until test
result is acceptable. If after 5 runs, test result still cannot meet specification, please check
reagent and tubing connection, and use “Back Flush” function in “Service” module to
eliminate block hole.
3.3 Blood sample collection
Blood sample collection is classified as venous blood and peripheral blood.
Warning: Do not touch blood sample, QC sample and calibration sample
directly. Handling of these objects shall comply with related
operation instruction.
3.3.1 Venous blood collection
Venous blood can be collected by using vacuum negative pressure tube or ordinary method
under atmospheric pressure, anticoagulant must be dropped to all venous blood collection
containers in advance, usually EDTA.K
2
.2H
2
O is adopted as anticoagulant, with content of
1.5-2.2mg/ml.
3.3.2 Peripheral blood collection
z Blood sampling position:
For adults, the inner side of middle finger or ring finger tips of left hand is better; for
children above half a year old, middle finger is better; for infant below half a year old,
blood is usually sampled from thumb or outer side of foot bottom.
z Blood sampling method:
It shall be carried out in accordance with peripheral blood collection standard of health
authority. Peripheral blood collection normally adopts local centesis; the typical
collection method is to pierce through finger end. Blood tube uses 20μL constant
volume blood tube or bullet blood tube. It is recommended to collect no less than 30μL
blood to facilitate double check.
During blood sampling, if blood flow stagnates, one may slightly press farther end of
wound, never force around piercing hole. Avoid tissue fluid mixed with blood, hampering
test analysis result accuracy.
3.3.3 Blood sample mixing
Before test, blood sample must be thoroughly shaken and mixed, the recommended method
is: shake up and down, rotate test tube 3-5min, do not shake too violently.
Blood sample to be tested can only stored at room temperature, test shall be done in 4h,
prolonged storage, or inferior mixing will influence test result accuracy.
3.4 Blood sample analysis
Prior to sample analysis, it is recommended to take one QC test of instrument, see detail
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