Sigma 6-16S - Centrifugation with Tubes for Mineral Oil

Laboratory Centrifuge Sigma 6-16S
6 Using the centrifuge
36 / 91
Version 07/2012, Rev. 1.22 of 08/05/2020 • sb
Translation of the original operating manual, part no. 0702002
A German blood bank works successfully using the following data:
1
Temperatur
e
1)
Speed
RCF
Timet
Acceleration curve
2)
Deceleration curve
Progra
m
°C
rpm
x g
min
no.
no.
22
3,750
4,544
7
20 (2 min)
12
1 Full blood
22
3,570
4,544
10
20 (2 min)
12
2 Full blood in 4fold bags
20
4,000
5,170
12
24 (3 min)
12
3 Plasma with buffy coat
22
3,750
4,544
9
20 (2 min)
12
4 Full blood
20
4,000
5,170
14
24 (3 min)
12
5 Plasma with buffy coat
22
1,094
384
7
21 (2 min)
11 or 2 min
6 Platelets with buffy coat
22
1,094
384
7
21 (2 min)
11 or 3.5min
7 Platelets with buffy coat
1)
only with special equipment heater
2)
Curves no. 20, 21 and 24 are freely programmable curves
NOTE
If the centrifuge does not reach the mentioned RCF values, the maximum
gravitational field must be set and the run time must be extended.
6.2.2.7 Centrifugation with tubes for mineral oil
In the previous ASTM standard, a maximum gravitational field of 800 x g is
required for the centrifugation with tubes for mineral oil. Practically, it is
possible to reach higher RCF-values with the rotor 11165 and the buckets
13113, 13114 or 13116..
CAUTION
The required gravitational field must not be exceeded.
Values above 800 x g increase the risk of glass breakage!
• Use sealing caps (part no. 17107).