Tuttnauer 2540 - Program 4: Liquid Sterilization (121°C)

Page 26 of 66 Pages
4.4 Program 4 (4 – Liquids)
For liquids when the manufacturer recommends autoclaving at
temperatures of 121ºC with no drying cycle.
Nominal Parameters
 Sterilization temperature: 121ºC(250ºF).
 Sterilization time: 20 minutes.
Operations Sequence
 Heating phase; The water enters the chamber and heats up until the
sterilization temperature is reached.
 Sterilization phase; temperature is maintained constant at preset level
for the sterilization time.
 Slow exhaust phase (Ex. Mode=4); steam is slowly exhausted from
the chamber, until it reaches the required temperature of around 85ºC
and the pressure equals the atmospheric pressure.
TIME
PRESSURE (kPa)
TEMPERATURE
Ambient Pressure
and Temperature
t1
t2
t3
t1 =
Heating
stage
t2 = Sterilization stage
t3 = Exhaust stage
= Pressure
= Temperature
220
kPa