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Waters 2545 Binary Gradient Module User Manual

Waters 2545 Binary Gradient Module
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Creating the inlet method 2-9
3. In the Solvent Names area, enter names for Solvent A and Solvent B. To
change solvent names, enter a name for solvent A in the label A box and
enter a name for Solvent B in the label B box.
Examples: acetonitrile, methanol, water.
4. In the Pressure Limits area, enter the low-pressure limit for the system
in the Low field. If the system pressure falls below this limit, the flow
stops and the LC Status error light turns red.
Range: 0 to 41,400 kPa (414 bar, 6000 psi). The default is 0 psi.
5. Enter the high-pressure limit for the system in the High field. If the
system exceeds this limit, the flow stops and the LC Status error light
turns red.
Range: 0 to 41,400 kPa (414 bar, 6000 psi). The default is 6000 psi.
6. In the Seal Wash Period field, enter the time interval between seal-wash
pump pulses. The seal wash lubricates the plunger and flushes away
solvent or precipitated salts forced past the plunger seal from the
high-pressure side of the solvent piston chamber.
Range: 0.5 to 9999 minutes. The default is 5 minutes.

Questions and Answers:

Waters 2545 Binary Gradient Module Specifications

General IconGeneral
TypeBinary Gradient Module
Gradient Composition Range0 to 100%
Flow Rate Precision<0.15% RSD
Gradient FormationHigh-pressure mixing
Gradient Accuracy±0.5%
Gradient Precision<0.15% RSD
Composition Accuracy±0.5%
Operating Temperature4°C to 40°C
Operating pH Range1 to 12
Weight13.6 kg (30 lb)

Summary

Setting Up the 2545 Binary Gradient Module

Installing the Binary Gradient Module

Steps and requirements for installing the hardware.

Unpacking and Inspecting

Procedures for safely unpacking and checking the module for damage.

Making Power Connections

Instructions for connecting the device to a power source.

Making Plumbing Connections

Guidance on connecting solvent inlet, seal-wash, and waste tubes.

Making Signal Connections

How to connect external devices and communication cables.

Preparing for Operation

Powering On

Steps to safely power on the 2545 Binary Gradient Module.

Creating the Inlet Method

Procedure for setting up the chromatographic method parameters.

Maintenance

Removing the Pump Head and Seal-Wash Assemblies

Step-by-step guide to disassembling pump components.

Replacing the Seals and O-ring

Detailed instructions for replacing seals and O-rings.

Replacing the Plunger

Procedure for replacing the plunger assembly.

Troubleshooting

Error Messages

Explanation and possible causes of common error messages.

Troubleshooting Hardware

Table of hardware issues, causes, and corrective actions.

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