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Waters 2545 Binary Gradient Module User Manual

Waters 2545 Binary Gradient Module
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4-2 Troubleshooting
System troubleshooting
Follow these basic steps to perform system troubleshooting:
1. Examine the system, checking the simple things first. Is something
obvious causing the problem? For example, is an instrument unplugged
or improperly connected?
2. Compare the current system operation with the way the system
operated before the problem started. To help you identify normal
operating conditions:
Record a map of your LC system (tubes and power connections).
Keep a daily log.
Run test chromatograms regularly.
Recommendation: Keep track of system parameters and the results of
your chromatography during normal operation. Troubleshooting is
easier if you know the typical conditions when the system is operating
correctly.
Example: If your system usually runs at nnn psi with a certain method,
is the system pressure currently in the same range, or drastically higher
(possibly caused by a clog) or lower (possibly caused by a leak)? Are
pressure fluctuations in the same pressure range as during normal
operation?
When your system is installed, and each time you develop a new method,
record the system conditions during normal operation.
3. Identify in the following order the symptom that varies from normal
system operation:
System pressure (high, low, or erratic)
Baseline (plumbing-related or detector-electronics-related)
Changes in peak retention time
Warning: To prevent injury, always observe good laboratory
practices when you handle solvents, change tubes, or operate the
binary gradient module. Know the physical and chemical properties
of the solvents you use. Refer to the Material Safety Data Sheets for
the solvents in use.

Questions and Answers:

Waters 2545 Binary Gradient Module Specifications

General IconGeneral
TypeBinary Gradient Module
Gradient Composition Range0 to 100%
Flow Rate Precision<0.15% RSD
Gradient FormationHigh-pressure mixing
Gradient Accuracy±0.5%
Gradient Precision<0.15% RSD
Composition Accuracy±0.5%
Operating Temperature4°C to 40°C
Operating pH Range1 to 12
Weight13.6 kg (30 lb)

Summary

Setting Up the 2545 Binary Gradient Module

Installing the Binary Gradient Module

Steps and requirements for installing the hardware.

Unpacking and Inspecting

Procedures for safely unpacking and checking the module for damage.

Making Power Connections

Instructions for connecting the device to a power source.

Making Plumbing Connections

Guidance on connecting solvent inlet, seal-wash, and waste tubes.

Making Signal Connections

How to connect external devices and communication cables.

Preparing for Operation

Powering On

Steps to safely power on the 2545 Binary Gradient Module.

Creating the Inlet Method

Procedure for setting up the chromatographic method parameters.

Maintenance

Removing the Pump Head and Seal-Wash Assemblies

Step-by-step guide to disassembling pump components.

Replacing the Seals and O-ring

Detailed instructions for replacing seals and O-rings.

Replacing the Plunger

Procedure for replacing the plunger assembly.

Troubleshooting

Error Messages

Explanation and possible causes of common error messages.

Troubleshooting Hardware

Table of hardware issues, causes, and corrective actions.

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