Table of Contents v
Safety considerations ......................................................................................... iii
Operating this device ......................................................................................... iv
1 Setting up the 2545 Binary Gradient Module .................................. 1-1
Features .............................................................................................................. 1-2
Installing the binary gradient module ........................................................ 1-4
Unpacking and inspecting .............................................................................. 1-6
Making power connections ............................................................................. 1-7
Making plumbing connections ...................................................................... 1-9
Making signal connections ........................................................................... 1-16
2 Preparing for operation ....................................................................... 2-1
Powering on ....................................................................................................... 2-2
Scanning connected instruments .................................................................. 2-3
Configuring 515 pumps .................................................................................... 2-6
Creating the inlet method ............................................................................... 2-8
Defining the gradient ..................................................................................... 2-10
Entering timed events ................................................................................... 2-14
Setting up analog signals .............................................................................. 2-16
Preparing solvent reservoirs ....................................................................... 2-17
3 Maintenance ............................................................................................ 3-1
Maintenance considerations .......................................................................... 3-2
Removing the pump head and seal-wash assemblies ............................... 3-4
Replacing the seals and O-ring .................................................................... 3-10
Table of Contents