Waters 3100 - Page 212

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D-4 Preparing samples for LC/MS system check with Empower software

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Copyright Notice2
Trademarks2
Customer Comments3
Safety Considerations4
Considerations Specific to the 3100 Detector4
Operating this Instrument7
Applicable Symbols7
Intended Use7
Calibrating7
Quality Control8
ISM Classification9
EC Authorized Representative9
Table of Contents11
Waters 3100 Detector18
Overview18
Overview19
Waters 3100 Detector20
Software and Data System20
Ionization Techniques and Source Probes21
Electrospray Ionization (ESI)21
Combined ESI and APCI (Esci)21
Atmospheric Pressure Chemical Ionization22
Atmospheric Pressure Photoionization22
Ion Optics23
MS Operating Modes24
Sample Inlet24
Vacuum System24
Rear Panel24
Intellistart Fluidics System26
Overview26
System Operation27
Preparing for Operation30
Starting the Instrument30
Configuring Intellistart32
Verifying the Instrument's State of Readiness32
Tuning and Calibration Information32
Monitoring the Instrument Leds33
Preparing the Intellistart Fluidics System34
Installing the Solvent Manifold Drip Tray34
Installing the Reservoir Bottles35
Diverter Valve Positions36
Purging the Infusion Syringe39
Rebooting the Instrument39
Rebooting the Instrument by Pressing the Reset Button39
Leaving the Mass Spectrometer Ready for Operation40
Emergency Instrument Shutdown40
ESI and Esci Modes of Operation42
Introduction42
Installing the ESI Probe42
Installing the Corona Pin46
Optimizing the ESI Probe for Esci Operation48
Removing the Corona Pin48
Removing the ESI Probe50
Optional APCI Mode of Operation54
Atmospheric Pressure Chemical Ionization54
Ionsabre APCI Probe54
Installing the Ionsabre APCI Probe55
Installing the Corona Pin58
Removing the Corona Pin58
Removing the Ionsabre APCI Probe59
Maintenance Procedures61
Maintenance Schedule63
Spare Parts65
Safety and Handling65
Preparing the Instrument for Work Performed on the Source66
Operating the Source Isolation Valve67
Removing O-Rings and Seals69
Cleaning the Instrument's Exterior70
Emptying the Exhaust Trap Bottle70
Emptying the Roughing Pump Exhaust Liquid Trap Bottle72
Gas Ballasting the Roughing Pump74
Gas Ballasting a Pump Fitted with a Screwdriver-Operated Gas Ballast Valve75
Gas Ballasting a Pump Fitted with a Handle-Operated Gas Ballast Valve76
Checking the Roughing Pump Oil Level77
Adding Oil to the Roughing Pump77
Cleaning the Source Components79
Cleaning the Sample Cone and Gas Cone79
Removing the Cone Gas Assembly from the Source79
Disassembling the Cone Gas Assembly82
Cleaning the Sample Cone and Gas Cone83
Assembling the Cone Gas Assembly86
Fitting the Cone Gas Assembly to the Source87
Cleaning the Ion Block, Isolation Valve, and Extraction Cone89
Removing the Ion Block Assembly from the Source Assembly89
Disassembling the Source Ion Block Assembly91
Cleaning the Ion Block, Isolation Valve, and Extraction Cone98
Assembling the Source Ion Block Assembly100
Fitting the Ion Block Assembly to the Source Assembly103
Cleaning the Source Hexapole Assembly105
Removing the Ion Block Assembly, Ion Block Support, and Hexapole from the Source Assembly105
Cleaning the Hexapole Assembly107
Fitting the Hexapole Assembly, PEEK Ion Block Support, and Ion Block Assembly to the Source Assembly110
Replacing the ESI Probe Tip112
Replacing the ESI Probe Sample Capillary113
Cleaning the Ionsabre APCI Probe Tip120
Replacing the Ionsabre APCI Probe Sample Capillary120
Removing the Existing Capillary120
Installing the New Capillary125
Cleaning or Replacing the Corona Pin129
Replacing the APCI Probe Heater130
Replacing the Ion Block Source Heater132
Replacing the Source Assembly Seals136
Removing the Source Enclosure from the Instrument136
Disassembling the Source Enclosure and Probe Adjuster Assembly139
Removing the Seals from the Source Enclosure and Probe Adjuster Assembly140
Fitting the New Source Enclosure and Probe Adjuster Assembly Seals143
Assembling the Probe Adjuster Assembly and Source Enclosure144
Fitting the Source Enclosure to the Instrument145
Maintaining the Instrument Air Filters147
Cleaning the Air Filter Inside the Instrument's Lower Bezel147
Replacing the Air Filter Inside the Lower Bezel148
Cleaning the Air Filter Behind the Source Probe149
Fit the Probe to the Source150
Replacing the Air Filter Behind the Source Probe151
Replacing the Roughing Pump Oil152
If You Are Fitting an ESI Probe, See "Installing the ESI Probe" on Page152
If You Are Fitting an Ionsabre APCI Probe, See "Installing the Ionsabre APCI Probe" on Page152
Start the Instrument (See "Starting the Instrument154
Replacing the Roughing Pump's Oil Demister Element155
A Safety Advisories162
Warning Symbols162
Task-Specific Hazard Warnings162
Specific Warnings163
Caution Symbol165
Warnings that Apply to All Waters Instruments165
Electrical and Handling Symbols171
Electrical Symbols171
Handling Symbols172
B External Connections174
External Wiring and Vacuum Connections174
Connecting the Oil-Filled Roughing Pump175
Making the Electrical Connections for a Roughing Pump with an External Relay Box180
Making the Electrical Connections for a Roughing Pump Without an External Relay Box181
Connecting the Oil-Free Roughing Pump182
Making the Electrical Connections for an Oil-Free Roughing Pump189
Connecting to the Nitrogen Gas Supply190
Connecting the Nitrogen Exhaust Line192
Connecting the Liquid Waste Line195
Connecting the Workstation197
Connecting Ethernet Cables198
I/O Signal Connectors198
Signal Connections200
Connecting to the Electricity Source203
C Materials of Construction and Compliant Solvents206
Preventing Contamination206
Items Exposed to Solvent206
Solvents Used to Prepare Mobile Phases207
Assembling Required Materials210
Preparing the Sulfadimethoxine Standard210
Storing the Solutions211
Using the Solution in an LC/MS System Check Run211
Index213

Waters 3100 - Page 212

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