June 28, 2016, 715003956 Rev. C
Page 73
C.3.2 Solvents used to prepare mobile phases
The table below lists solvents and reagents used commonly to prepare mobile phase and, in some
cases, act as sample diluents. They are not expected to adversely affect the performance of the
materials shown in the table on page 72.
Note: The list of solvents and chemicals and their associated concentrations shown below
presents the levels which have been tested, assessed, or both, for chemical compatibility with the
materials of construction. It is not a recommendation for optimal electrospray sensitivity.
Mobile-phase component concentration affects electrospray efficiency and sensitivity. Therefore,
keep modifier and buffer concentrations to the lowest levels that maintain chromatographic
resolution.
O-rings
Viton
®
or
PTFE (polytetrafluoroethylene)-
encapsulated Viton
Solvent waste/leak management Fluorinated ethyline polymer (FEP) tubing
Source enclosure Aluminium
Waste bottle Polypropylene
Pumping Block gaskets FEPM
Waste bottle push-in fittings NBR (nitrile butadiene rubber), SST (stainless
steel), PBT (polybutylene terephthalate), and
POM (polyoxymethylene)
Notice: For LC/MS operation, do not prepare mobile phases using normal-phase
solvents, like hexane. Such solvents adversely affect the performance of the materials
named in the list of items exposed to solvents, on page 72, and should not be used
except at or below the concentrations specified. In the event the solvents are used, at
reduced concentrations, as additives to mobile phases or as sample diluents, you must
evaluate the consequent compatibility issues.
Table C–2: Solvents used to prepare mobile phases (KAD)
Solution Concentration
Water 100%
Methanol 100%
Acetonitrile 100%
Ethanol 100%
Isopropanol 100%
Propanol 100%
Formic acid ≤0.5%
Acetic acid ≤1%
Trifluoroacetic acid (TFA) ≤0.2%
Table C–1: Items exposed to solvent (KAD) (Continued)
Item Material
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