3-18 Troubleshooting, Testing, and Maintenance
Reproducibility errors Solvent not properly
degassed or sparged
Degas or sparge
solvent.
Incorrect chemistry Check chemistry.
Incorrect integration Incorrect integration.
Injector problem Troubleshoot injector.
Baseline drift, rapid Column not
equilibrated
Equilibrate column.
Detector not allowed to
warm up
Allow detector to warm
up until baseline is
stable (time varies
based on wavelength
and sensitivity).
Solvent contaminated Use fresh solvent.
Solvent not properly
degassed (rapid or slow
drift)
Degas solvent.
Flow fluctuations (rapid
or slow drift
Troubleshoot fluid
handling unit.
Incorrect wavelength
for solvent
Use correct wavelength
and solvent (make sure
solvent does not have a
UV absorbance at the
wavelength used).
Chromatography troubleshooting (Continued)
Symptom Possible cause Corrective action
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