Getting started 1-21
– Methods specifying vial numbers
– Number of injections from each vial
– Injection volume
The controller can contain 15 sets of Method tables. Each set of tables can
contain these individual tables:
• Gradient – Includes up to 15 operational steps that define the varying
solvent composition, flow rate, time range, and rate of change for each
phase of the gradient.
• Event – Includes up to 15 operational steps to control output signals to
external devices, internal alarms, sparge rate, and linking operation to
other tables.
• Detector – Includes up to 15 operational steps to change wavelength
and attenuation during a run.
You can create a Method table, a time-based program that can include up to
48 individual steps. Each step accesses a set of Gradient/Event/Detector
tables. These are among the tasks the Method table can perform:
• Equilibrating the system for a specific period of time
• Switching solvents
• Purging the autoinjector
• Purging the 2414 RI Detector flow cell
• Performing a series of small-scale or large-scale injections
• Cleaning up following buffer use
• Lowering the system flow rate after an analysis
Having programmed the Method table, you can instruct the system to start at
any step in the process.
Screen layout
The figure below shows the screens associated with the system’s PowerLine
configuration.
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