Waters HPLC - Page 88

3-4 Troubleshooting, Testing, and Maintenance
Pump cavitating Solvent reservoirs
positioned below solvent
proportioning valve
Place solvents on bench
beside fluid handling
unit.
Leak in tubing
connections or loose
fittings prior to inlet
check valve, causing
aspiration of air (fluid
leak may not be visible)
Eliminate leak, tighten
fittings, replace ferrules.
Solvent inlet filter
restricting flow
Clean or replace filters.
RI detector leaking
internally or from
purge port
Cell damaged –
maximum flow rate or
pressure of RI cell
exceeded
Do not exceed 5 mL/min
(2414, 2410, and 410
Detectors). Do not exceed
100 psi for any Waters RI
detector.
Replace detector flow cell
if necessary (see detector
operator’s guide).
Peak retention times
halved
Incorrect pump head
volume entered on
PUMP
CONFIGURATION
screen
For PrepLC operation
with 1000-µL pump
heads, enter 1000 for
pump head volume on
the PUMP
CONFIGURATION
screen.
Unable to enter a
flow rate above
150 mL/min
Incorrect pump head
volume entered on
PUMP
CONFIGURATION
screen
For operation with
1000-µL pump heads,
enter 1000 for pump
head volume on the
PUMP
CONFIGURATION
screen.
Pulsing flow from
waste line
Dirty check valve(s) Clean check valve(s).
General PrepLC troubleshooting (Continued)
Symptom Possible cause Corrective action