NanoFlow source 3-21
7. Carefully remove the new borosilicate glass capillary from its case by
lifting vertically while pressing on the foam with two fingers.
8. Load sample into the capillary using a fused silica syringe needle or a
GELoader tip, minimizing any bubbles between the capillary tip and the
sample.
Recommendation: When using a GELoader tip, break the glass
capillary in half, scoring it with a fused silica cutter so that the
GELoader can reach the capillary’s tip.
9. Thread the knurled nut and approximately 5 mm of conductive
elastomer over the blunt end of the capillary
10. Fit the capillary into the holder (probe).
11. Finger-tighten the nut so that 5 mm of glass capillary protrude from its
end.
Tip: Measure the protrusion from the end of the nut to the shoulder of
the glass capillary.
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