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Audience and purpose
This guide is for operators of varying levels of experience. It gives an overview
of the instrument and explains how to prepare it for operation, switch between
modes of operation, and maintain it.
Intended use of the Xevo TQD
Waters designed the Xevo
®
TQD for use as a research tool to accurately,
reproducibly, and robustly quantify target compounds present at the lowest
possible levels in highly complex sample matrices. It is not for use in
diagnostic procedures.
When fitted with Waters APCI, APGC, APPI, ASAP, ESCi
®
, NanoFlow™ ESI,
or TRIZAIC™ UPLC
®
options, or optional third-party sources (DART
®
, DESI,
or LDTD™), the Xevo TQD does not comply with the European Union In Vitro
Diagnostic Device Directive 98/79/EC.
Calibrating
To calibrate LC systems, follow acceptable calibration methods using at least
five standards to generate a standard curve. The concentration range for
standards must cover the entire range of quality-control samples, typical
specimens, and atypical specimens.
To calibrate the Xevo TQD, consult the instrument’s online Help system.
Quality control
Routinely run three QC samples that represent subnormal, normal, and
above-normal levels of a compound. Ensure that QC sample results fall within
an acceptable range, and evaluate precision from day to day and run to run.
Data collected when QC samples are out of range are sometimes invalid. Do
not report these data until you are certain that the instrument performs
satisfactorily.
When analyzing samples from a complex matrix such as soil, tissue,
serum/plasma, whole blood, and other sources, note that the matrix
components can adversely affect LC/MS results, enhancing or suppressing
ionization. To minimize these matrix effects, adopt the following measures:
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